Search for:
hicstatistics
Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines
Endometriosis is a persistent inflammatory illness which more and more impacts younger girls underneath 35 years of age and results in subfertility even infertility. Evaluation of the cytotoxic impact of zinc(II) niflumato advanced with neocuproine ([Zn(neo)(nif)2] or Zn-Nif) on immortalized human endometriotic cell line (12Z) and on management immortalized human endometrial stromal cell line (hTERT) was carried out utilizing xCELLigence know-how for roughly 72 h following the remedy with Zn-Nif in addition to cell viability Trypan Blue Assay. 12Z cell line proliferated extra slowly in comparison with unaffected cells, whereas hTERT cells didn’t present related conduct after remedy.
The advanced in all probability reduces the impact of pro-inflammatory pathways as a result of impact of NSAID, whereas presence of zinc may scale back the extent of ROS and regulate ER2 ranges and MMP exercise. The noticed results and excessive selectivity for quickly proliferating cells with elevated inflammatory exercise recommend an excellent prognosis of profitable lower of endometriosis stage with this advanced.

Mineralized polyplexes for gene supply: Enchancment of transfection effectivity as a consequence of calcium incubation and never mineralization

Gene remedy is an rising discipline wherein nucleic acids are used to manage protein expression. The need of delivering nucleic acids to particular cell varieties and intracellular websites calls for the usage of extremely specialised gene carriers. As a provider modification method, mineralization has been efficiently used to change viral and non-viral carriers, offering new properties that in the end intention to extend the transfection effectivity. Nonetheless, for the particular case of polyplexes utilized in gene remedy, latest literature exhibits that interplay with calcium, a elementary step of mineralization, could be efficient to extend transfection effectivity, leaving an ambiguity about of the function of mineralization for this sort of gene carriers.
To reply this query and to disclose the properties chargeable for rising transfection effectivity, we mineralized poly(aspartic acid) coated polyplexes at various CaCl2 and Na3PO4 concentrations, and evaluated the resultant carriers for physicochemical and morphological traits, in addition to transfection and supply effectivity with MC3T3-E1 mouse osteoblastic cells.
We discovered that each mineralization and calcium incubation positively affected the transfection effectivity and uptake of polyplexes in MC3T3-E1 cells. Nonetheless, this impact originated from the properties achieved by polyplexes after the calcium incubation step which might be maintained after mineralization, together with particle measurement enhance, improved pDNA binding, and adjustment of zeta potential. Contemplating that mineralization generally is a longer course of than calcium incubation, we discover that calcium incubation could be enough and most popular if improved transfection effectivity in vitro is the one impact desired.
Staphylococcus epidermidis has been not too long ago acknowledged as an rising nosocomial pathogen. There are considerations over the rising virulence potential of this commensal as a result of capabilities of transferring cell genetic parts to Staphylococcus aureus by way of staphylococcal chromosomal cassette (SCCmec) and the carefully associated arginine catabolic cell factor (ACME) and the copper and mercury resistance island (COMER). The potential pathogenicity of S. epidermidis, significantly from blood stream infections, has been poorly investigated. On this research, 24 S. epidermidis remoted from blood stream infections from Oman had been investigated utilizing entire genome sequence evaluation. Core genome phylogenetic bushes revealed one third of the isolates belong to the multidrug resistance ST-2.
Genomic evaluation unraveled a typical incidence of SCCmec sort IV and ACME factor predominantly sort I organized in a composite island. The genetic composition of ACME was extremely variable amongst isolates of identical or totally different STs. The COMER-like island was absent in all of our isolates. Lowered copper susceptibility was noticed amongst isolates of ST-2 and ACME sort I, adopted by ACME sort V. In conclusion, on this work, we establish a prevalent incidence of extremely variable ACME parts in several hospital STs of S. epidermidis in Oman, thus strongly suggesting the speculation that ACME varieties developed from carefully associated STs.
hicstatistics
hicstatistics

Antibiotics, Multidrug-Resistant Micro organism, and Antibiotic Resistance Genes: Indicators of Contamination in Mangroves?

Multidrug-resistant micro organism and antibiotic resistance genes will be monitored as indicators of contamination in a number of environments. Mangroves are among the many most efficient ecosystems, and though they are often resilient to the motion of local weather phenomena, their equilibrium will be affected by anthropogenic actions. Relating to the presence and persistence of multidrug-resistant micro organism in mangroves, it is not uncommon to suppose that this ecosystem can operate as a reservoir, which might disperse the antibiotic resistance capability to human pathogens, or function a filter to remove drug-resistant genes.
The attainable affect of anthropogenic actions carried out close to mangroves is reviewed, together with wastewater remedy, meals manufacturing techniques, leisure, and tourism. Hostile results of antibiotic resistance genes or multidrug-resistant micro organism, thought-about as rising contaminants, haven’t been reported but in mangroves. Quite the opposite, mangrove ecosystems generally is a pure technique to remove antibiotics, antibiotic-resistant micro organism, and even antibiotic-resistant genes from the surroundings.
Though mangroves’ function in reducing antibiotics and antibiotic resistance genes from the surroundings is being proposed, the mechanisms by which these vegetation scale back these rising contaminants haven’t been elucidated and want additional research. Moreover, additional analysis is required on the consequences of antibiotics and antibiotic-resistant micro organism in mangroves to generate an evaluation of the human contribution to the degradation of this particular ecosystem in addition to to outline if these contaminants can be utilized as indicators of contamination in mangrove ecosystems.
Irrigation water is effectively generally known as potential supply of pathogens in recent produce. Nonetheless, its function in transferring antibiotic resistance determinants is much less effectively investigated. Due to this fact, we analyzed the contribution of floor and faucet water to the resistome of overhead-irrigated chive vegetation. Area-grown chive was irrigated with both floor water (R-system) or faucet water (D-system), from planting to reap. Water alongside the 2 irrigation chains in addition to the respective vegetation had been repeatedly sampled and screened for 264 antibiotic resistance genes (ARGs) and cell genetic parts (MGEs), utilizing high-capacity qPCR.
Differentially plentiful (DA) ARGs had been decided by evaluating the 2 systems. On R-chive, β-lactam ARGs, multidrug-resistance (MDR) determinants, and MGEs had been most plentiful, whereas D-chive featured DA ARGs from the vancomycin class. Variety and variety of DA ARGs was the best on younger chives, strongly diminished at harvest, and elevated once more on the finish of shelf life.
Most ARGs extremely enriched on R- in comparison with D-chive had been additionally enriched in R- in comparison with D-sprinkler water, indicating that water performed a significant function in ARG enrichment. Of be aware, blaKPC was detected at excessive ranges in floor water and chive. We conclude that water high quality considerably impacts the resistome of the irrigated produce.
hicstatistics
Postharvest Treatment of Hydrogen Sulfide Delays the Softening of Chilean Strawberry Fruit by Downregulating the Expression of Key Genes Involved in Pectin Catabolism
Hydrogen sulfide (H2S) performs a number of physiological roles in crops. Regardless of the proof, the position of H2S on cell wall disassembly and its implications on fleshy fruit firmness stays unknown. On this work, the impact of H2S therapy on the shelf-life, cell wall polymers and cell wall modifying-related gene expression of Chilean strawberry (Fragaria chiloensis) fruit was examined throughout postharvest storage.
The therapy with H2S extended the shelf-life of fruit by an impact of optimum dose. Fruit handled with 0.2 mM H2S maintained considerably increased fruit firmness than non-treated fruit, lowering its decay and tripling its shelf-life. Moreover, H2S therapy delays pectin degradation all through the storage interval and considerably downregulated the expression of genes encoding for pectinases, corresponding to polygalacturonase, pectate lyase, and expansin.
This proof means that H2S as a gasotransmitter prolongs the post-harvest shelf-life of the fruit and prevents its quick softening fee by a downregulation of the expression of key pectinase genes, which results in a decreased pectin degradation.

The Lipid-Modulating Impact of Tangeretin on the Inhibition of Angiopoietin-like 3 (ANGPTL3) Gene Expression via Regulation of LXRα Activation in Hepatic Cells

The extreme accumulation of TG-rich lipoproteins (TGRLs) in plasma is related to dyslipidemia and atherosclerotic cardiovascular illnesses (ASCVDs). Tangeretin is a bioactive pentamethoxyflavone primarily present in citrus peels, and it has been reported to guard towards hyperlipidemia, diabetes, and weight problems. The intention of this examine was to research the lipid-modulating results and the underlying mechanisms of tangeretin motion in hepatic cells.
Transcriptome and bioinformatics analyses with the Gene Ontology (GO) database confirmed that tangeretin considerably regulated a set of 13 differentially expressed genes (DEGs) related to the regulation of lipoprotein lipase (LPL) exercise. Amongst these DEGs, angiopoietin-like 3 (ANGPTL3), an important inhibitor of LPL catalytic exercise that regulates TGRL metabolism in plasma, was markedly downregulated by tangeretin. We demonstrated that tangeretin considerably inhibited the mRNA expression of ANGPTL3 in HepG2 and Huh-7 cells. Tangeretin therapy of hepatic cells additionally lowered the degrees of each intracellular and secreted ANGPTL3 proteins. Furthermore, we discovered that inhibition of ANGPTL3 manufacturing by tangeretin augmented LPL exercise.
We additional demonstrated that the transcriptional exercise of the ANGPTL3 promoter was considerably attenuated by tangeretin, and we recognized a DNA aspect positioned between the -250 and -121 positions that responded to tangeretin. Moreover, we discovered that tangeretin didn’t alter the degrees of the nuclear liver X receptor α (LXRα) protein, an important transcription issue that binds to the tangeretin-responsive aspect, however it could actually counteract LXRα-mediated ANGPTL3 transcription.
On the premise of molecular docking evaluation, tangeretin was predicted to bind to the ligand-binding area of LXRα, which might lead to suppression of LXRα activation. Our findings help the speculation that tangeretin exerts a lipid-lowering impact by modulating the LXRα-ANGPTL3-LPL pathway, and thus, it may be used as a possible phytochemical for the prevention or therapy of dyslipidemia.

Factors of View on the Instruments for Genome/Gene Modifying

Theoretically, a DNA sequence-specific recognition protein that may distinguish a DNA sequence equal to or greater than 16 bp might be distinctive to mammalian genomes. Lengthy-sequence-specific nucleases, corresponding to naturally occurring Homing Endonucleases and artificially engineered ZFN, TALEN, and Cas9-sgRNA, have been developed and broadly utilized in genome enhancing. In distinction to different counterparts, which acknowledge DNA goal websites by the protein moieties themselves, Cas9 makes use of a single-guide RNA (sgRNA) as a template for DNA goal recognition.
As a result of simplicity in designing and synthesizing a sgRNA for a goal website, Cas9-sgRNA has turn into essentially the most present software for genome enhancing. Furthermore, the RNA-guided DNA recognition exercise of Cas9-sgRNA is impartial of each of the nuclease actions of it on the complementary strand by the HNH area and the non-complementary strand by the RuvC area, and HNH nuclease exercise null mutant (H840A) and RuvC nuclease exercise null mutant (D10A) had been recognized.
In accompaniment with the sgRNA, Cas9, Cas9(D10A), Cas9(H840A), and Cas9(D10A, H840A) can be utilized to attain double strand breakage, complementary strand breakage, non-complementary strand breakage, and no breakage on-target website, respectively. Based mostly on such distinctive traits, many engineered enzyme actions, corresponding to DNA methylation, histone methylation, histone acetylation, cytidine deamination, adenine deamination, and primer-directed mutation, might be launched inside or across the goal website.
In an effort to stop off-targeting by the lasting expression of Cas9 derivatives, numerous transient expression strategies, together with the direct supply of Cas9-sgRNA riboprotein, had been developed. The difficulty of biosafety is indispensable in in vivo purposes; Cas9-sgRNA packaged into virus-like particles or extracellular vesicles have been designed and a few in vivo therapeutic trials have been reported.
hicstatistics
hicstatistics

De Novo Transcriptome Meeting, Practical Annotation, and Transcriptome Dynamics Analyses Reveal Stress Tolerance Genes in Mangrove Tree ( Bruguiera gymnorhiza)

Their excessive adaptability to troublesome coastal circumstances makes mangrove bushes a precious useful resource and an fascinating mannequin system for understanding the molecular mechanisms underlying stress tolerance and adaptation of crops to the annoying environmental circumstances. On this examine, we used RNA sequencing (RNA-Seq) for de novo assembling and characterizing the Bruguiera gymnorhiza (L.) Lamk leaf transcriptome. B. gymnorhiza is likely one of the most generally distributed mangrove species from the largest household of mangroves; Rhizophoraceae. The de novo meeting was adopted by useful annotations and identification of particular person transcripts and gene households which are concerned in abiotic stress response.
We then in contrast the genome-wide expression profiles between two populations of B. gymnorhiza, rising beneath totally different ranges of stress, of their pure habitats. One inhabitants residing in excessive salinity atmosphere, within the shore of the Pacific Ocean- Japan, and the opposite inhabitants residing about one kilometre farther from the ocean, and subsequent to the estuary of a river; in much less saline and extra brackish situation.
Many genes concerned in response to salt and osmotic stress, confirmed elevated expression ranges in bushes rising subsequent to the ocean in excessive salinity situation. Validation of those genes might contribute to future salt-resistance analysis in mangroves and different woody crops. Moreover, the sequences and transcriptome information offered on this examine are precious scientific assets for future comparative transcriptome analysis in crops rising beneath annoying circumstances.

 

 

hicstatistics
The Effect of Water Deficit on Two Greek Vitis vinifera L. Cultivars: Physiology, Grape Composition and Gene Expression during Berry Development
Vegetation are uncovered to quite a few abiotic stresses. Drought might be crucial of them and determines crop distribution world wide. Grapevine is taken into account to be a drought-resilient species, historically overlaying semiarid areas. Furthermore, within the case of grapevine, average water deficit is thought to enhance the standard traits of grape berries and subsequently wine composition.
Nonetheless, in opposition to the backdrop of local weather change, vines are anticipated to expertise sustained water deficits which may very well be detrimental to each grape high quality and yield. The affect of water deficit on two Greek Vitis vinifera L. cultivars, ‘Agiorgitiko’ and ‘Assyrtiko’, was investigated in the course of the 2019 and 2020 vintages. Vine physiology measurements in irrigated and non-irrigated crops have been carried out at three time-points all through berry improvement (inexperienced berry, veraison and harvest).
Berry progress and composition have been examined throughout ripening. In response to the outcomes, water deficit resulted in diminished berry dimension and elevated ranges of soluble sugars, whole phenols and anthocyanins. The expression profile of particular genes, recognized to manage grape coloration, aroma and taste was altered by water availability throughout maturation in a cultivar-specific method.
In settlement with the elevated focus of phenolic compounds attributable to water deficit, genes of the phenylpropanoid pathway within the red-skinned Agiorgitiko exhibited greater expression ranges and earlier up-regulation than within the white Assyrtiko. The expression profile of the opposite genes throughout maturation or in response to water deficit was relied on the classic.

Figuring out Clinicopathological Elements Related to Oncotype DX  21-Gene Recurrence Rating: A Actual-World Retrospective Cohort Examine of Breast Most cancers Sufferers in Quebec Metropolis, Canada

Gene expression profiling assessments such because the Oncotype DX (ODX) 21-gene recurrence rating (RS) assay is more and more utilized in medical follow to foretell the chance of recurrence and assist therapy planning for early-stage breast most cancers (BC). Nonetheless, this check has some disadvantages similar to a excessive value and an extended turnaround time to get outcomes, which can result in disparities in entry. We intention to determine clinicopathological components related to ODX RS in girls with early-stage BC. We performed a retrospective cohort examine of girls recognized within the medical database of the Deschênes-Fabia Breast Illness Middle of Quebec Metropolis College, Canada. Our pattern consists of 425 girls identified with early-stage BC who’ve obtained an ODX RS between January 2011 and April 2015. The ODX RS has been categorized into three ranges as initially outlined: low (0-17), intermediate (18-30), and excessive (>30). The imply RS was 17.8 (SD = 9.2).
Univariate analyses and multinomial logistic regressions have been carried out to determine components related to intermediate and excessive RS in contrast with low RS. A complete of 237 (55.8%) sufferers had low RS, 148 (34.8%) had intermediate RS, and 40 (9.4%) had excessive RS. Girls with progesterone receptor (PR)-negative (ORs starting from 3.51 to 10.34) and histologic grade II (ORs starting from 3.16 to 23.04) tumors have been constantly extra prone to have intermediate or excessive RS than low RS. Comparable patterns of associations have been noticed when the RS was categorised utilizing redefined thresholds from (i.e., from the TAILORx examine or dichotomized).
This examine gives proof suggesting that histologic grade and PR standing are predictive components for intermediate or excessive RS in girls with early-stage BC. If these outcomes are confirmed in future research, contemplating these clinicopathological components may spare girls the necessity to get such a check earlier than the start of a doable adjuvant remedy. This feature may very well be thought of in settings the place the price of testing is a matter.
hicstatistics
hicstatistics

Gene Evaluation, Cloning, and Heterologous Expression of Protease from a Micromycete Aspergillus ochraceus Able to Activating Protein C of Blood Plasma

Micromycetes are recognized to secrete quite a few enzymes of biotechnological and medical potential. Fibrinolytic protease-activator of protein C (PAPC) of blood plasma from micromycete Aspergillus ochraceus VKM-F4104D was obtained in recombinant type utilising the bacterial expression system. This enzyme, which belongs to the proteinase-Ok-like proteases, is much like the proteases encoded within the genomes of Aspergillus fumigatus ATCC MYA-4609, A. oryzae ATCC 42149 and A. flavus 28.
Mature PAPC-4104 is 282 amino acids lengthy, preceded by the 101-amino acid propeptide crucial for correct folding and maturation. The recombinant protease was similar to the native enzyme from micromycete by way of its organic properties, together with a capability to hydrolyse substrates of activated protein C (pGlu-Professional-Arg-pNA) and issue Xa (Z-D-Arg-Gly-Arg-pNA) in conjugant reactions with human blood plasma.
Subsequently, recombinant PAPC-4104 can probably be utilized in medication, veterinary science, diagnostics, and different purposes. Useful annotation of unknown operate genes reveals unidentified features that may improve our understanding of advanced genome communications. A typical method for inferring gene operate entails the ortholog-based methodology. Nonetheless, genetic information alone are sometimes not sufficient to offer data for operate annotation.
Thus, integrating different sources of knowledge can probably improve the potential for retrieving annotations. Community-based strategies are environment friendly strategies for exploring interactions amongst genes and can be utilized for practical inference. On this examine, we current an evaluation framework for inferring the features of Plasmodium falciparum genes primarily based on connection profiles in a heterogeneous community between human and Plasmodium falciparum proteins. These profiles have been fed right into a hybrid deep studying algorithm to foretell the orthologs of unknown operate genes.
The outcomes present excessive efficiency of the mannequin’s predictions, with an AUC of 0.89. 100 and twenty-one predicted pairs with excessive prediction scores have been chosen for inferring the features utilizing statistical enrichment evaluation. Utilizing this methodology, PF3D7_1248700 and PF3D7_0401800 have been discovered to be concerned with muscle contraction and striated muscle tissue improvement, whereas PF3D7_1303800 and PF3D7_1201000 have been discovered to be associated to protein dephosphorylation. In conclusion, combining a heterogeneous community and a hybrid deep studying approach can enable us to determine unknown gene features of malaria parasites. This method is generalized and will be utilized to different illnesses that improve the sphere of biomedical science.

 

hicstatistics
The impact of anionic polymers on gene delivery: how composition and assembly help evading the toxicity-efficiency dilemma
Cationic polymers have been broadly studied for non-viral gene supply on account of their means to bind genetic materials and to work together with mobile membranes. Nonetheless, their charged nature carries the danger of elevated cytotoxicity and interplay with serum proteins, limiting their potential in vivo utility. Due to this fact, hydrophilic or anionic shielding polymers are utilized to counteract these results. Herein, a sequence of micelle-forming and micelle-shielding polymers have been synthesized through RAFT polymerization.
The copolymer poly[(n-butyl acrylate)-b-(2-(dimethyl amino)ethyl acrylamide)] (P(nBA-b-DMAEAm)) was assembled into cationic micelles and totally different shielding polymers have been utilized, i.e., poly(acrylic acid) (PAA), poly(4-acryloyl morpholine) (PNAM) or P(NAM-b-AA) block copolymer. These techniques have been in comparison with a triblock terpolymer micelle comprising PAA as the center block. The assemblies have been investigated relating to their morphology, interplay with pDNA, cytotoxicity, transfection effectivity, polyplex uptake and endosomal escape.
The bare cationic micelle exhibited superior transfection effectivity, however elevated cytotoxicity. The addition of protecting polymers led to diminished toxicity. Particularly, the triblock terpolymer micelle satisfied with excessive cell viability and no important loss in effectivity. The very best shielding impact was achieved by layering micelles with P(NAM-b-AA) supporting the colloidal stability at impartial zeta potential and utterly restoring cell viability whereas sustaining reasonable transfection efficiencies. The excessive potential of this micelle-layer-combination for gene supply was illustrated for the primary time.

Genetic Evaluation, Inhabitants Construction, and Characterisation of Multidrug-Resistant Klebsiella pneumoniae from the Al-Hofuf Area of Saudi Arabia

Multidrug-resistant Klebsiella pneumoniae (MDR-KP) is a significant public well being drawback that’s globally related to illness outbreaks and excessive mortality charges. Because the world seeks options to such pathogens, international and regional surveillance is required. The goal of the current research was to look at the antimicrobial susceptibility sample and clonal relatedness of Klebsiella pneumoniae isolates collected for a interval of three years by way of pulse discipline gel electrophoresis (PFGE).
Isolate IDs, antimicrobial assays, ESBL-production, and minimal inhibitory concentrations (MICs) have been examined with the Vitek 2 Compact Automated System. IDs have been confirmed by 16S rRNA gene sequencing, with the ensuing sequences being deposited in NCBI databases. DNA was extracted and resistance genes have been detected by PCR amplification with applicable primers. Isolates have been intensive (31%) and multidrug-resistant (65%).
Pulsotype clusters grouped the isolates into 22 band profiles that confirmed no particular sample with phenotypes. Of the isolates, 98% have been ESBL-KP, 69% have been carbapenem-resistant Enterobacteriaceae (CRE) strains, and 72.5% comprised the carriage of two MBLs (SIM and IMP). Integrons (ISAba1, ISAba2, and IS18) have been detected in 69% of the MDR-KP. Moreover, OXA-23 was detected in 67% of the isolates. This research subsequently demonstrates clonal range amongst scientific Ok. pneumoniae, confirming that this bacterium has entry to an infinite pool of genes that confer excessive resistance-developing potential.

Full Genome Sequencing of Leptospira interrogans Isolates from Malaysia Reveals Large Genome Rearrangement however Excessive Conservation of Virulence-Related Genes

The power of Leptospirae to persist in environments and animal hosts however to trigger clinically extremely variable illness in people has made leptospirosis the most typical zoonotic illness. Contemplating the paucity of knowledge on variation in full genomes of human pathogenic Leptospirae, we’ve used a mix of Single Molecule Actual-Time (SMRT) and Illumina sequencing to acquire full genome sequences of six human scientific L. interrogans isolates from Malaysia.
  • All six contained the bigger (4.28-4.56 Mb) and smaller (0.34-0.395 Mb) chromosome typical of human pathogenic Leptospirae and 0-7 plasmids. Solely 24% of the plasmid sequences could possibly be matched to databases. We recognized a chromosomal core genome of 3318 coding sequences and strain-specific accent genomes of 49-179 coding sequences.
  • These sequences enabled detailed genomic pressure typing (Genome BLAST Distance Phylogeny, DNA-DNA hybridization, and multi locus sequence typing) and phylogenetic classification (whole-genome SNP genotyping). Regardless that there was some shared synteny and collinearity throughout the six genomes, there was proof of main genome rearrangement, doubtless pushed by horizontal gene switch and homologous recombination.
  • Cellular genetic parts have been recognized in all strains in extremely various numbers, together with within the rfb locus, which defines serogroups and contributes to immune escape and pathogenesis. Then again, there was excessive conservation of virulence-associated genes together with these referring to sialic acid, alginate, and lipid A biosynthesis.
  • These findings counsel (i) that the antigenic variation, adaption to numerous host environments, and broad spectrum of virulence of L. interrogans are partially on account of a excessive diploma of genomic plasticity and (ii) that human pathogenic strains keep a core set of genes required for virulence.
hicstatistics
hicstatistics

Genomic Analyses of Penicillium Species Have Revealed Patulin and Citrinin Gene Clusters and Novel Loci Concerned in Oxylipin Manufacturing

Blue mildew of apple is attributable to a number of totally different Penicillium species, amongst which P. expansum and P. solitum are essentially the most ceaselessly remoted. P. expansum is essentially the most aggressive species, and P. solitum could be very weak when infecting apple fruit throughout storage. On this research, we report full genomic analyses of three totally different Penicillium species: P. expansum R21 and P. crustosum NJ1, remoted from saved apple fruit; and Pmaximae 113, remoted in 2013 from a flooded house in New Jersey, USA, within the aftermath of Hurricane Sandy. Patulin and citrinin gene cluster analyses defined the shortage of patulin manufacturing in NJ1 in comparison with R21 and lack of citrinin manufacturing in all three strains.
Drosophila bioassay demonstrated that volatiles emitted by Psolitum SA and Ppolonicum RS1 have been extra poisonous than these from Pexpansum and P. crustosum strains (R27, R11, R21, G10, and R19). The toxicity was hypothesized to be associated to manufacturing of eight-carbon oxylipins. Putative lipoxygenase genes have been recognized in Pexpansum and Pmaximae strains, however not in Pcrustosum. Our information will present a greater understanding of Penicillium spp. complicated secondary metabolic capabilities, particularly regarding the genetic bases of mycotoxins and poisonous VOCs.
Pig-to-human xenotransplantation appears to be the response to the up to date scarcity of tissue/organ donors. Sadly, the phylogenetic distance between pig and human implies hyperacute xenograft rejection. On this research, we examined the speculation that combining expression of human α1,2-fucosyltransferase (hFUT2) and α-galactosidase A (hGLA) genes would enable for removing of this impediment in porcine transgenic epidermal keratinocytes (PEKs). We sought to find out not solely the expression profiles of recombinant human α1,2-fucosyltransferase (rhα1,2-FT) and α-galactosidase A (rhα-Gal A) proteins, but in addition the relative abundance (RA) of Galα1→3Gal epitopes within the PEKs stemming from not solely hFUT2 or hGLA single-transgenic and hFUT2×hGLA double-transgenic pigs. Our confocal microscopy and Western blotting analyses revealed that each rhα1,2-FT and rhα-Gal A enzymes have been overabundantly expressed in respective transgenic PEK traces.
Furthermore, the semiquantitative ranges of Galα1→3Gal epitope that have been assessed by lectin fluorescence and lectin blotting have been discovered to be considerably diminished in every variant of genetically modified PEK line as in comparison with these noticed within the management nontransgenic PEKs. Notably, the bi-transgenic PEKs have been characterised by considerably lessened (however nonetheless detectable) RAs of Galα1→3Gal epitopes as in comparison with these recognized for each varieties of mono-transgenic PEK traces. Moreover, our present investigation confirmed that the coexpression of two protecting transgenes gave rise to enhanced abrogation of Galα→3Gal epitopes in hFUT2×hGLA double-transgenic PEKs.
To summarize, detailed estimation of semiquantitative profiles for human α-1,2-FT and α-Gal A proteins adopted by identification of the extent of abrogating the abundance of Galα1→3Gal epitopes within the ex vivo expanded PEKs stemming from mono- and bi-transgenic pigs have been discovered to be a sine qua non situation for effectively ex situ defending steady traces of skin-derived somatic cells inevitable in additional research.
The latter is because of be targeted on figuring out epigenomic reprogrammability of single- or double-transgenic cell nuclei inherited from grownup cutaneous keratinocytes in porcine nuclear-transferred oocytes and corresponding cloned embryos. To our data, this idea was proven to signify a very new strategy designed to generate and multiply genetically reworked pigs by somatic cell cloning for the wants of reconstructive drugs and dermoplasty-mediated tissue engineering of human integumentary system.
hicstatistics
A Gene-Expression Predictor for Efficacy of Induction Chemotherapy in Locoregionally Advanced Nasopharyngeal Carcinoma

Background: Induction chemotherapy (IC) adopted by concurrent chemoradiotherapy is the mainstay therapy for sufferers with locoregionally superior nasopharyngeal carcinoma. Nonetheless, some sufferers acquire little profit and expertise pointless poisonousities from IC. We supposed to develop a gene-expression signature that may establish beneficiaries of IC.

 

Strategies: We screened chemosensitivity-related genes by evaluating gene-expression profiles of sufferers with short-term tumor response or nonresponse to IC (n = 95) utilizing microarray evaluation. Chemosensitivity-related genes had been quantified by digital expression profiling in a coaching cohort (n = 342) to acquire a gene signature. We then validated this gene signature within the scientific trial cohort (n = 187) and an exterior impartial cohort (n = 240). Assessments of statistical significance are 2-sided.

 

Outcomes: We recognized 43 chemosensitivity-related genes related to the short-term tumor response to IC. Within the coaching cohort, a 6-gene signature was developed that was extremely correct at predicting the short-term tumor response to IC (space below the curve [AUC] = 0.87, sensitivity = 87.5%, specificity = 75.6%).

We additional discovered that IC conferred failure-free survival advantages solely in sufferers within the profit group (hazard ratio [HR] = 0.54, 95% confidence interval [CI] = 0.34 to 0.87; P = .01) and never on these within the no-benefit group (HR = 1.25, 95% CI = 0.62 to 2.51; P = .53). Within the scientific trial cohort, the 6-gene signature was additionally extremely correct at predicting the tumor response (AUC = 0.82, sensitivity = 87.5%, specificity = 71.8%) and indicated failure-free survival advantages. Within the exterior impartial cohort, comparable outcomes had been noticed.

 

Conclusions: The 6-gene signature will help choose beneficiaries of IC and lay a basis for a extra individualized therapeutic technique for locoregionally superior nasopharyngeal carcinoma sufferers.

 

Genetic Occasions and Signaling Mechanisms Underlying Schwann Cell Destiny in Improvement and Most cancers

 

On this assessment, we describe Schwann cell improvement from embryonic neural crest cells to terminally differentiated myelinated and nonmyelinated mature Schwann cells. We deal with the genetic drivers and signaling mechanisms mediating choices to proliferate versus differentiate throughout Schwann cell improvement, highlighting pathways that overlap with Schwann cell improvement and are dysregulated in tumorigenesis.

We conclude by contemplating how our data of the occasions underlying Schwann cell improvement and mouse fashions of schwannoma, neurofibroma, and malignant peripheral nerve sheath tumor can inform novel therapeutic methods for sufferers with cancers derived from Schwann cell lineages.

The event and controversy of aggressive endogenous RNA speculation in non-coding genes

As a momentous post-transcriptional regulator, microRNAs (miRNAs) are attracting increasingly more consideration. The classical miRNAs regulated mechanism exhibits it binds to the targets’ 3’UTR thus play the function in post-transcription.

In the meantime, single miRNA can goal a number of genes, so these ought to compete to bind that miRNA. Vice versa, single gene can sponge mass of miRNAs as nicely. Thus the aggressive endogenous RNAs (ceRNAs) speculation was put ahead in 2011.

The ceRNA speculation has made big achievements, particularly in non-coding genes, which together with lengthy non-coding RNAs (lncRNAs), circle RNAs (circRNAs) and pseudogenes, even viral transcripts. It additionally contributed enormously to epigenetics improvement.

Nonetheless, an growing variety of controversies have occurred with applause. Primarily based on this example, this assessment introduces one thing in element in regards to the ceRNAs speculation achieved in lncRNAs,circRNAs, pseudogenes and viral transcripts, respectively. In the meantime, it additionally covers controversy of the ceRNAs speculation

hicstatistics
hicstatistics

Conserved Patterns in Developmental Processes and Phases, Somewhat than Genes, Unite the Extremely Divergent Bilateria 

  • Bilateria are the predominant clade of animals on Earth. Regardless of having developed all kinds of physique plans and developmental modes, they’re characterised by frequent morphological traits. By default, researchers have tried to hyperlink clade-particular genes to those traits, thus distinguishing bilaterians from non-bilaterians, by their gene content material.
  • Right here we argue that it’s relatively organic processes that unite Bilateria and set them aside from their non-bilaterian sisters, with a much less advanced physique morphology. To check this speculation, we in contrast proteomes of bilaterian and non-bilaterian species in an elaborate computational pipeline, aiming to seek for a set of bilaterian-specific genes. Regardless of the restricted confidence of their bilaterian specificity, we however detected Bilateria-specific practical and developmental patterns within the sub-set of genes conserved in distantly associated Bilateria.

 

  • Utilizing a novel multi-species GO-enrichment technique, we decided the practical repertoire of genes which might be extensively conserved amongst Bilateria. Analyzing expression profiles in three very distantly associated mannequin species- melanogasterD. rerioand C. elegans-we discover attribute peaks at comparable phases of improvement and a delayed onset of expression in embryos. Particularly, the expression of the conserved genes seems to peak on the phylotypic stage of various bilaterian phyla.

 

  • In abstract, our examine illustrate how improvement connects distantly associated Bilateria after thousands and thousands of years of divergence, pointing to processes doubtlessly separating them from non-bilaterians. We argue that evolutionary biologists ought to return from a purely gene-centric view of evolution and place extra deal with analyzing and defining conserved developmental processes and durations.

The efficacy of chemotherapeutic drug mixtures could also be predicted by concordance of gene response to the one brokers

Figuring out the expression of genes in response to completely different courses of chemotherapeutic medicine could permit for a greater understanding as to which can be used successfully together. Within the current examine, the human colorectal most cancers cell line HCT116 was cultured with equi-active concentrations of a sequence of anti-cancer brokers.

Gene expression profiles had been then measured by whole-genome microarray. Though every drug induced a singular signature of gene expression in tumour cells, there have been marked similarities between sure medicine, even in these from completely different courses. For instance, the antimalarial agent artesunate and the platinum-containing alkylating agent, oxaliplatin, produced a really comparable mRNA expression sample in HCT116 cells with ~14,000 genes being affected by the 2 medicine in the identical method.

Moreover, the general correlation of gene responses between two brokers may predict whether or not their use together would result in a higher or lesser impact on cell quantity, decided experimentally, than predicted by single agent experiments. The outcomes indicated that even when working by means of completely different mechanisms, combining medicine that provoke an identical transcriptional response could represent the most suitable choice for figuring out drug-combination methods for the therapy of most cancers.

 

Human Pim-3 Oncogene (PIM3) ELISA Kit

DLR-PIM3-Hu-96T 96T
EUR 725
  • Should the Human Pim-3 Oncogene (PIM3) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Pim-3 Oncogene (PIM3) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Pim-3 Oncogene (PIM3) ELISA Kit

RDR-PIM3-Hu-48Tests 48 Tests
EUR 589

Human Pim-3 Oncogene (PIM3) ELISA Kit

RDR-PIM3-Hu-96Tests 96 Tests
EUR 820

Human Pim-3 Oncogene (PIM3) ELISA Kit

RD-PIM3-Hu-48Tests 48 Tests
EUR 563

Human Pim-3 Oncogene (PIM3) ELISA Kit

RD-PIM3-Hu-96Tests 96 Tests
EUR 783

Pim-3 Oncogene (PIM3) Antibody

20-abx131576
  • EUR 453.00
  • EUR 133.00
  • EUR 1316.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Pim-3 Oncogene (PIM3) Antibody

20-abx174068
  • EUR 926.00
  • EUR 467.00
  • 1 mg
  • 200 ug
  • Please enquire.

Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human)

4-PAN637Hu01
  • EUR 262.00
  • EUR 2747.00
  • EUR 679.00
  • EUR 331.00
  • EUR 220.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM3 (Met1~Leu326)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3)

Recombinant Pim-3 Oncogene (PIM3)

4-RPN637Hu01
  • EUR 467.36
  • EUR 228.00
  • EUR 1477.60
  • EUR 559.20
  • EUR 1018.40
  • EUR 376.00
  • EUR 3544.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q86V86
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 39.6kDa
  • Isoelectric Point: Inquire
Description: Recombinant Human Pim-3 Oncogene expressed in: E.coli

Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), APC

4-PAN637Hu01-APC
  • EUR 368.00
  • EUR 3599.00
  • EUR 993.00
  • EUR 472.00
  • EUR 229.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM3 (Met1~Leu326)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with APC.

Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), Biotinylated

4-PAN637Hu01-Biotin
  • EUR 328.00
  • EUR 2697.00
  • EUR 786.00
  • EUR 404.00
  • EUR 226.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM3 (Met1~Leu326)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with Biotin.

Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), Cy3

4-PAN637Hu01-Cy3
  • EUR 449.00
  • EUR 4757.00
  • EUR 1283.00
  • EUR 588.00
  • EUR 264.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM3 (Met1~Leu326)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with Cy3.

Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), FITC

4-PAN637Hu01-FITC
  • EUR 314.00
  • EUR 2899.00
  • EUR 814.00
  • EUR 397.00
  • EUR 203.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM3 (Met1~Leu326)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with FITC.

Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), HRP

4-PAN637Hu01-HRP
  • EUR 335.00
  • EUR 3135.00
  • EUR 877.00
  • EUR 426.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM3 (Met1~Leu326)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with HRP.

Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), PE

4-PAN637Hu01-PE
  • EUR 314.00
  • EUR 2899.00
  • EUR 814.00
  • EUR 397.00
  • EUR 203.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM3 (Met1~Leu326)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with PE.

Human Pim-3 Oncogene (PIM3) Protein

20-abx650812
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human), APC-Cy7

4-PAN637Hu01-APC-Cy7
  • EUR 616.00
  • EUR 7078.00
  • EUR 1867.00
  • EUR 824.00
  • EUR 338.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM3 (Met1~Leu326)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3). This antibody is labeled with APC-Cy7.

Human Pim-3 Oncogene (PIM3)ELISA Kit

201-12-2347 96 tests
EUR 440
  • This Pim-3 Oncogene ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Pim-3 Oncogene (PIM3) CLIA Kit

20-abx190020
  • EUR 7911.00
  • EUR 4215.00
  • EUR 973.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Human Pim-3 Oncogene (PIM3) ELISA Kit

20-abx152746
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Human Pim-3 Oncogene(PIM3)ELISA Kit

QY-E04696 96T
EUR 361

Rat Pim-3 Oncogene(PIM3)ELISA Kit

QY-E10537 96T
EUR 400

Human Pim-3 Oncogene (PIM3)CLIA Kit

SCN637Hu-10x96wellstestplate 10x96-wells test plate
EUR 5647.8
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3) in tissue homogenates and other biological fluids.

Human Pim-3 Oncogene (PIM3)CLIA Kit

SCN637Hu-1x48wellstestplate 1x48-wells test plate
EUR 552.76
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3) in tissue homogenates and other biological fluids.

Human Pim-3 Oncogene (PIM3)CLIA Kit

SCN637Hu-1x96wellstestplate 1x96-wells test plate
EUR 746.8
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3) in tissue homogenates and other biological fluids.

Human Pim-3 Oncogene (PIM3)CLIA Kit

SCN637Hu-5x96wellstestplate 5x96-wells test plate
EUR 3060.6
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3) in tissue homogenates and other biological fluids.

Human Pim-3 Oncogene (PIM3) CLIA Kit

4-SCN637Hu
  • EUR 5698.00
  • EUR 3061.00
  • EUR 747.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Pim-3 Oncogene Clia kit. Alternative names of the recognized antigen: Serine/threonine-protein kinase pim-3
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Pim-3 Oncogene (PIM3)tissue homogenates and other biological fluids

Mouse Pim-3 Oncogene(PIM3)ELISA Kit

QY-E21186 96T
EUR 361

Human Pim-3 Oncogene (PIM3) ELISA Kit

SEN637Hu-10x96wellstestplate 10x96-wells test plate
EUR 5189.65
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-3 Oncogene (PIM3) in serum, plasma, tissue homogenates and other biological fluids.

Human Pim-3 Oncogene (PIM3) ELISA Kit

SEN637Hu-1x48wellstestplate 1x48-wells test plate
EUR 515.03
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-3 Oncogene (PIM3) in serum, plasma, tissue homogenates and other biological fluids.

Human Pim-3 Oncogene (PIM3) ELISA Kit

SEN637Hu-1x96wellstestplate 1x96-wells test plate
EUR 692.9
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-3 Oncogene (PIM3) in serum, plasma, tissue homogenates and other biological fluids.

Human Pim-3 Oncogene (PIM3) ELISA Kit

SEN637Hu-5x96wellstestplate 5x96-wells test plate
EUR 2818.05
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-3 Oncogene (PIM3) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-3 Oncogene (PIM3) in serum, plasma, tissue homogenates and other biological fluids.

Human Pim-3 Oncogene (PIM3) ELISA Kit

4-SEN637Hu
  • EUR 5240.00
  • EUR 2769.00
  • EUR 693.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Pim-3 Oncogene elisa. Alternative names of the recognized antigen: Serine/threonine-protein kinase pim-3
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Pim-3 Oncogene (PIM3) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

ELISA kit for Human PIM3 (Pim-3 Oncogene)

ELK6491 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pim-3 Oncogene (PIM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pim-3 Oncoge
  • Show more
Description: A sandwich ELISA kit for detection of Pim-3 Oncogene from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 280

Serine/Threonine-Protein Kinase Pim-3 (PIM3) Antibody

20-abx214125
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Serine/Threonine-Protein Kinase Pim-3 (PIM3) Antibody

20-abx241699
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Serine/threonine-Protein Kinase Pim-3 (PIM3) Antibody

20-abx318212
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Rabbit Pim 1 Oncogene ELISA kit

E04P0753-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Pim 1 Oncogene ELISA kit

E04P0753-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Pim 1 Oncogene ELISA kit

E04P0753-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Serine/threonine-Protein Kinase Pim-3 (PIM3) Antibody (HRP)

20-abx315070
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Serine/threonine-Protein Kinase Pim-3 (PIM3) Antibody (FITC)

20-abx315071
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Serine/threonine-Protein Kinase Pim-3 (PIM3) Antibody (Biotin)

20-abx315072
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse)

4-PAC578Mu01
  • EUR 251.00
  • EUR 2576.00
  • EUR 640.00
  • EUR 316.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1)

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat)

4-PAC578Ra01
  • EUR 259.00
  • EUR 2708.00
  • EUR 670.00
  • EUR 328.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr38~Leu177)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1)

Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human)

4-PAP797Hu01
  • EUR 262.00
  • EUR 2747.00
  • EUR 679.00
  • EUR 331.00
  • EUR 220.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM2 (Leu82~Glu291)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2)

Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse)

4-PAP797Mu01
  • EUR 266.00
  • EUR 2813.00
  • EUR 694.00
  • EUR 337.00
  • EUR 222.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM2 (Gly98~Cys319)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2)

Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat)

4-PAP797Ra01
  • EUR 275.00
  • EUR 2958.00
  • EUR 727.00
  • EUR 350.00
  • EUR 226.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM2 (Leu34~Glu292)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2)

Pim-1 Oncogene (PIM1) Antibody

20-abx101233
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Pim-1 Oncogene (PIM1) Antibody

20-abx101234
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Pim-1 Oncogene (PIM1) Antibody

20-abx101235
  • EUR 453.00
  • EUR 133.00
  • EUR 1302.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.

Pim-2 Oncogene (PIM2) Antibody

20-abx101236
  • EUR 467.00
  • EUR 133.00
  • EUR 1344.00
  • EUR 634.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Pim-2 Oncogene (PIM2) Antibody

20-abx101237
  • EUR 481.00
  • EUR 133.00
  • EUR 1414.00
  • EUR 662.00
  • EUR 356.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Pim-2 Oncogene (PIM2) Antibody

20-abx129066
  • EUR 453.00
  • EUR 133.00
  • EUR 1316.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Pim-1 Oncogene (PIM1) Antibody

abx146417-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.

Pim-1 Oncogene (PIM1) Antibody

20-abx174066
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug
  • Please enquire.

Pim-2 Oncogene (PIM2) Antibody

20-abx174067
  • EUR 926.00
  • EUR 467.00
  • 1 mg
  • 200 ug
  • Please enquire.

Pim-2 Oncogene (PIM2) Antibody

abx236456-100ug 100 ug
EUR 551
  • Shipped within 5-12 working days.

Pim-2 Oncogene (PIM2) Antibody

abx431508-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

Pim-2 Oncogene (PIM2) Antibody

abx433133-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

Pim-2 Oncogene (PIM2) Antibody

abx433134-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

Anti-PIM3 antibody

STJ119424 100 µl
EUR 277
Description: The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), APC

4-PAC578Mu01-APC
  • EUR 351.00
  • EUR 3365.00
  • EUR 935.00
  • EUR 449.00
  • EUR 222.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with APC.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), Biotinylated

4-PAC578Mu01-Biotin
  • EUR 316.00
  • EUR 2526.00
  • EUR 744.00
  • EUR 387.00
  • EUR 221.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with Biotin.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), Cy3

4-PAC578Mu01-Cy3
  • EUR 427.00
  • EUR 4445.00
  • EUR 1205.00
  • EUR 557.00
  • EUR 254.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with Cy3.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), FITC

4-PAC578Mu01-FITC
  • EUR 301.00
  • EUR 2712.00
  • EUR 768.00
  • EUR 379.00
  • EUR 197.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with FITC.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), HRP

4-PAC578Mu01-HRP
  • EUR 321.00
  • EUR 2933.00
  • EUR 827.00
  • EUR 405.00
  • EUR 209.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with HRP.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), PE

4-PAC578Mu01-PE
  • EUR 301.00
  • EUR 2712.00
  • EUR 768.00
  • EUR 379.00
  • EUR 197.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with PE.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), APC

4-PAC578Ra01-APC
  • EUR 364.00
  • EUR 3545.00
  • EUR 980.00
  • EUR 467.00
  • EUR 227.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr38~Leu177)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with APC.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), Biotinylated

4-PAC578Ra01-Biotin
  • EUR 325.00
  • EUR 2658.00
  • EUR 777.00
  • EUR 400.00
  • EUR 225.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr38~Leu177)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Biotin.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), Cy3

4-PAC578Ra01-Cy3
  • EUR 444.00
  • EUR 4685.00
  • EUR 1265.00
  • EUR 581.00
  • EUR 261.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr38~Leu177)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Cy3.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), FITC

4-PAC578Ra01-FITC
  • EUR 311.00
  • EUR 2856.00
  • EUR 804.00
  • EUR 393.00
  • EUR 202.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr38~Leu177)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with FITC.

Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), HRP

4-PAC578Ra01-HRP
  • EUR 332.00
  • EUR 3089.00
  • EUR 866.00
  • EUR 421.00
  • EUR 213.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr38~Leu177)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with HRP.
hicstatistics
Novel Gene Rearrangement and the Full Mitochondrial Genome of Cynoglossusmonopus: Insights into the Envolution of the Family Cynoglossidae (Pleuronectiformes)

Cynoglossusmonopus, a small benthic fish, belongs to the Cynoglossidae, Pleuronectiformes. It was not usually studied due to its low abundance and cryptical life-style. With the intention to understand the mitochondrial genome and the phylogeny in Cynoglossidae, all the mitogenome of C. monopus has been sequenced and analyzed for the first time. The whole dimension is 16,425 bp, generally containing 37 genes with novel gene rearrangements.

 

The tRNA-Gln gene is inverted from the sunshine to the heavy strand and translocated from the downstream of tRNA-Ile gene to its upstream. The administration space (CR) translocated downstream to the three’-end of ND1 gene adjoining to inverted to tRNA-Gln and left a 24 bptrace fragment inside the genuine place.

 

The phylogenetic timber had been reconstructed by Bayesian inference (BI) and most probability (ML) methods based mostly totally on the mitogenomic info of 32 tonguefish species and two outgroups. The outcomes assist the idea that Cynoglossidae is a monophyletic group and level out that C. monopus has the closest phylogenetic relationship with C. puncticeps.

 

By combining fossil info and mitogenome info, the time-calibrated evolutionary tree of households Cynoglossidae and Soleidae was firstly provided, and it was indicated that Cynoglossidae and Soleidae had been differentiated from each other all through Paleogene, and the evolutionary strategy of family Cynoglossidae coated the Quaternary, Neogene and Paleogene durations.

 

 

The Full Chloroplast Genome of Arabidopsis thaliana Isolated in Korea (Brassicaceae): An Investigation of Intraspecific Variations of the Chloroplast Genome of Korean A. thaliana

 

 

Arabidopsis thaliana (L.) Heynh. is a model organism of plant molecular biology. Higher than 1,700 full genome sequences have been sequenced, nonetheless no Korean isolate genomes have been sequenced to this point though many A. thaliana isolated in Japan and China have been sequenced. To understand the genetic background of Korean pure A. thaliana (named as 180404IB4),

 

we provided its full chloroplast genome, which is 154,464 bp prolonged and has Four subregions: 85,164 bp of giant single copy (LSC) and 17,781 bp of small single copy (SSC) areas are separated by 26,257 bp of inverted repeat (IRs) areas along with 130 genes (85 protein-coding genes, eight rRNAs, and 37 tRNAs). Fifty single nucleotide polymorphisms (SNPs) and 14 insertion and deletions (INDELs) are acknowledged between 180404IB4 and Col0.

 

In addition to, 101 SSRs and 42 extendedSSRs had been acknowledged on the Korean A. thaliana chloroplast genome, indicating an identical number of SSRs on the remaining 5 chloroplast genomes with a alternative of sequence variations in the direction of the SSR space. A nucleotide vary analysis revealed two extraordinarily variable areas on A. thaliana chloroplast genomes. Phylogenetic timber with three additional chloroplast genomes of East Asian pure isolates current that Korean and Chinese language language pure isolates are clustered collectively, whereas two Japanese isolates aren’t clustered, suggesting the need for additional investigations of the chloroplast genomes of East Asian isolates.

 

Loci acknowledged by a genome-wide affiliation study of carotid artery stenosis inside the eMERGE neighborhood

 

Carotid artery atherosclerotic sickness (CAAD) is a risk subject for stroke. We used a genome-wide affiliation (GWAS) technique to seek out genetic variants associated to CAAD in members inside the digital Medical Data and Genomics (eMERGE) Group. We acknowledged grownup CAAD situations with unilateral or bilateral carotid artery stenosis and controls with out proof of stenosis from digital nicely being info at eight eMERGE web sites. We carried out GWAS with a model adjusting for age, intercourse, study web site, and genetic principal components of ancestry.

 

In eMERGE we found 1793 CAAD situations and 17,958 controls. Two loci reached genome-wide significance, on chr6 in LPA (rs10455872, odds ratio [OR] (95% confidence interval [CI]) = 1.50 (1.30-1.73), p = 2.1 × 10-8 ) and on chr7, an intergenic single nucleotide variant (SNV; rs6952610, OR (95% CI) = 1.25 (1.16-1.36), p = 4.3 × 10-8 ). The chr7 affiliation remained very important inside the presence of the LPA SNV as a covariate. The LPA SNV was moreover associated to

 

coronary coronary coronary heart sickness (CHD; 4199 situations and 11,679 controls) on this study (OR (95% CI) = 1.27 (1.13-1.43), p = 5 × 10-5 ) nonetheless the chr7 SNV was not (OR (95% CI) = 1.03 (0.97-1.09), p = .37). Every variants replicated in UK Biobank. Elevated lipoprotein(a) concentrations ([Lp(a)]) and LPA variants associated to elevated [Lp(a)] have beforehand been associated to CAAD and CHD, along with rs10455872. With digital nicely being file phenotypes in eMERGE and UKB, we replicated a beforehand acknowledged affiliation and acknowledged a novel locus associated to CAAD.

Immunophenotypic Landscape and Prognosis of Diffuse Large B-Cell Lymphoma with MYC/BCL2 Double Expression: An Analysis of A Prospectively Immunoprofiled Cohort

Diffuse large B-cell lymphoma (DLBCL) patients with MYC/BCL2 double expression (DE) show poor prognosis and their clinical outcomes after R-CHOP therapy vary immensely. We investigated the prognostic value of DE in aggressive B-cell lymphoma patients (n = 461), including those with DLBCL (n = 417) and high-grade B-cell lymphoma (HGBL; n = 44), in a prospectively immunoprofiled cohort. DE was observed in 27.8% of DLBCLs and 43.2% of HGBLs (P = 0.058). DE-DLBCL patients were older (P = 0.040) and more frequently exhibited elevated serum LDH levels (P = 0.002), higher international prognostic index (IPI; P = 0.042), non-germinal-center B-cell phenotype (P < 0.001), and poor response to therapy (P = 0.042) compared to non-DE-DLBCL patients.
In R-CHOP-treated DLBCL patients, DE status predicted poor PFS and OS independently of IPI (P < 0.001 for both). Additionally, in DE-DLBCL patients, older age (>60 years; P = 0.017), involvement of ³2 extranodal sites (P = 0.021), bone marrow involvement (P = 0.001), high IPI (P = 0.017), CD10 expression (P = 0.006), poor performance status (P = 0.028), and elevated LDH levels (P < 0.001) were significantly associated with poor OS.
Notably, DE-DLBCL patients with normal LDH levels exhibited similar PFS and OS to those of patients with non-DE-DLBCL. Our findings suggest that MYC/BCL2 DE predicts poor prognosis in DLBCL. Risk stratification of DE-DLBCL patients based on LDH levels may guide clinical decision-making for DE-DLBCL patients.
hicstatistics
hicstatistics

Resveratrol relieves chlorothalonil-induced apoptosis and necroptosis through miR-15a/Bcl2-A20 axis in fish kidney cells

Chlorothalonil (CT) is a commonly used fungicide and its excessive application seriously threatens aquatic life and human health. Resveratrol (RSV) is a natural polyphenol and can be used as a therapeutic and preventive agent for the treatment of various diseases. To explore the toxic mechanism of CT exposure on fish kidney cell, as well as the alleviation effect of RSV, we established CT poisoning and/or RSV treatment fish kidney cell models. Ctenopharyngodon idellus kidney (CIK) cell line was treated with CT (5 μg/L) and/or RSV (10 μM) for 48 h.
The results showed that CT exposure activated cytochromeP450s (CYPs) including CYP1A1, CYP1B1 and CYP1C, caused malondialdehyde (MDA) accumulation, inhibited glutathione (GSH) levels and glutathione peroxidase (GPX) activities, increased the expression of miR-15a and downregulated BCL2 and TNFα-induced protein 3 (TNFAIP3, A20), triggered mitochondrial pathway mediated apoptosis and receptor interacting serine/threonine kinase (RIP)-dependent necroptosis in CIK cells.
However, cell death under CT exposure could be relieved by RSV treatment through inhibiting the expression of CYP1 family genes and restoring miR-15a/BCL2-A20 axis disorders. Overall, we conclude that RSV could relieve CT-induced apoptosis and necroptosis through miR-15a/Bcl2-A20 axis in CIK cells. These results enrich the toxicological mechanisms of the CT and confirm that RSV can be used as a potential antidote for CT poisoning.

Mouse Caspase 12 (CASP12) ELISA Kit

RD-CASP12-Mu-96Tests 96 Tests
EUR 677

Anti-Caspase-12/CASP12 Antibody

PA2103 100ug/vial
EUR 334

Caspase 12 (CASP12) Antibody

abx026563-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Caspase 12 (CASP12) Antibody

abx026563-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Caspase 12 (CASP12) Antibody

abx216233-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

Caspase 12 (CASP12) Antibody

20-abx213378
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Caspase 12 (CASP12) Antibody

20-abx210324
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Caspase 12 (CASP12) Antibody

20-abx100899
  • EUR 425.00
  • EUR 133.00
  • EUR 1177.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Caspase 12 (CASP12) Antibody

20-abx111421
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

Caspase 12 (CASP12) Antibody

20-abx129818
  • EUR 411.00
  • EUR 133.00
  • EUR 1135.00
  • EUR 551.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Caspase 12 (Casp12) Antibody

20-abx318989
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Caspase 12 (CASP12) Antibody

20-abx324638
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

Caspase 12 (CASP12) Antibody

20-abx318255
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse)

4-PAA682Mu01
  • EUR 236.00
  • EUR 2338.00
  • EUR 586.00
  • EUR 294.00
  • EUR 209.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Phe413)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Caspase 12 (CASP12)

Recombinant Caspase 12 (CASP12)

4-RPA682Mu01
  • EUR 467.36
  • EUR 228.00
  • EUR 1477.60
  • EUR 559.20
  • EUR 1018.40
  • EUR 376.00
  • EUR 3544.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: O08736
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 79.6kDa
  • Isoelectric Point: 6.1
Description: Recombinant Mouse Caspase 12 expressed in: E.coli

Recombinant Caspase 12 (CASP12)

4-RPA682Ra01
  • EUR 494.24
  • EUR 235.00
  • EUR 1578.40
  • EUR 592.80
  • EUR 1085.60
  • EUR 394.00
  • EUR 3796.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q920D5
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 31.4kDa
  • Isoelectric Point: 5.1
Description: Recombinant Rat Caspase 12 expressed in: E.coli

Caspase 12 (Casp12) Antibody (HRP)

20-abx318990
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Caspase 12 (Casp12) Antibody (FITC)

20-abx318991
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Caspase 12 (Casp12) Antibody (Biotin)

20-abx318992
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Caspase 12 (CASP12) Antibody (HRP)

20-abx316303
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Caspase 12 (CASP12) Antibody (FITC)

20-abx316304
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Caspase 12 (CASP12) Antibody (Biotin)

20-abx316305
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse), APC

4-PAA682Mu01-APC
  • EUR 329.00
  • EUR 3041.00
  • EUR 854.00
  • EUR 416.00
  • EUR 212.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Phe413)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Caspase 12 (CASP12). This antibody is labeled with APC.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse), Biotinylated

4-PAA682Mu01-Biotin
  • EUR 299.00
  • EUR 2288.00
  • EUR 684.00
  • EUR 363.00
  • EUR 214.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Phe413)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Caspase 12 (CASP12). This antibody is labeled with Biotin.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse), Cy3

4-PAA682Mu01-Cy3
  • EUR 397.00
  • EUR 4013.00
  • EUR 1097.00
  • EUR 513.00
  • EUR 241.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Phe413)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Caspase 12 (CASP12). This antibody is labeled with Cy3.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse), FITC

4-PAA682Mu01-FITC
  • EUR 283.00
  • EUR 2452.00
  • EUR 703.00
  • EUR 353.00
  • EUR 189.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Phe413)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Caspase 12 (CASP12). This antibody is labeled with FITC.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse), HRP

4-PAA682Mu01-HRP
  • EUR 302.00
  • EUR 2652.00
  • EUR 756.00
  • EUR 377.00
  • EUR 200.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Phe413)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Caspase 12 (CASP12). This antibody is labeled with HRP.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse), PE

4-PAA682Mu01-PE
  • EUR 283.00
  • EUR 2452.00
  • EUR 703.00
  • EUR 353.00
  • EUR 189.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Phe413)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Caspase 12 (CASP12). This antibody is labeled with PE.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse, Rat)

4-PAA682Ra01
  • EUR 243.00
  • EUR 2457.00
  • EUR 613.00
  • EUR 305.00
  • EUR 212.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Thr244)
  • Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Mouse, Rat Caspase 12 (CASP12)

Mouse Caspase 12 (CASP12) Protein

20-abx167225
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Rat Caspase 12 (CASP12) Protein

20-abx065753
  • EUR 690.00
  • EUR 286.00
  • EUR 2124.00
  • EUR 815.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse), APC-Cy7

4-PAA682Mu01-APC-Cy7
  • EUR 538.00
  • EUR 5962.00
  • EUR 1588.00
  • EUR 713.00
  • EUR 304.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Phe413)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Caspase 12 (CASP12). This antibody is labeled with APC-Cy7.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse, Rat), APC

4-PAA682Ra01-APC
  • EUR 340.00
  • EUR 3203.00
  • EUR 894.00
  • EUR 432.00
  • EUR 217.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Thr244)
  • Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Mouse, Rat Caspase 12 (CASP12). This antibody is labeled with APC.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse, Rat), Biotinylated

4-PAA682Ra01-Biotin
  • EUR 307.00
  • EUR 2407.00
  • EUR 714.00
  • EUR 375.00
  • EUR 217.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Thr244)
  • Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Mouse, Rat Caspase 12 (CASP12). This antibody is labeled with Biotin.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse, Rat), Cy3

4-PAA682Ra01-Cy3
  • EUR 411.00
  • EUR 4229.00
  • EUR 1151.00
  • EUR 535.00
  • EUR 248.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Thr244)
  • Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Mouse, Rat Caspase 12 (CASP12). This antibody is labeled with Cy3.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse, Rat), FITC

4-PAA682Ra01-FITC
  • EUR 292.00
  • EUR 2582.00
  • EUR 735.00
  • EUR 366.00
  • EUR 194.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Thr244)
  • Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Mouse, Rat Caspase 12 (CASP12). This antibody is labeled with FITC.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse, Rat), HRP

4-PAA682Ra01-HRP
  • EUR 311.00
  • EUR 2792.00
  • EUR 791.00
  • EUR 391.00
  • EUR 205.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Thr244)
  • Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Mouse, Rat Caspase 12 (CASP12). This antibody is labeled with HRP.

Caspase 12 (CASP12) Polyclonal Antibody (Mouse, Rat), PE

4-PAA682Ra01-PE
  • EUR 292.00
  • EUR 2582.00
  • EUR 735.00
  • EUR 366.00
  • EUR 194.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Thr244)
  • Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Mouse, Rat Caspase 12 (CASP12). This antibody is labeled with PE.

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 349

Rat Casp12/ Caspase-12 ELISA Kit

E0156Ra 1 Kit
EUR 571

Mouse Casp12/ Caspase-12 ELISA Kit

E0211Mo 1 Kit
EUR 571

Human Caspase 12 (CASP12) CLIA Kit

abx196504-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Rat Caspase 12 (CASP12) CLIA Kit

abx196787-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.

Mouse Caspase 12 (CASP12) ELISA Kit

abx254790-96tests 96 tests
EUR 668
  • Shipped within 5-12 working days.

Rat Caspase 12 (CASP12) ELISA Kit

abx256323-96tests 96 tests
EUR 786
  • Shipped within 5-12 working days.

Mouse Caspase 12 (CASP12) ELISA Kit

20-abx258452
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Human Caspase 12 (CASP12) ELISA Kit

20-abx259273
  • EUR 6642.00
  • EUR 3542.00
  • EUR 825.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-12 working days.

Human Caspase- 12, CASP12 ELISA KIT

ELI-02372h 96 Tests
EUR 824

Mouse Caspase- 12, Casp12 ELISA KIT

ELI-02373m 96 Tests
EUR 865

Rat Caspase 12 (CASP12) ELISA Kit

abx353550-96tests 96 tests
EUR 786
  • Shipped within 5-12 working days.

Human Caspase 12 (CASP12) ELISA Kit

abx358592-96tests 96 tests
EUR 707
  • Shipped within 5-12 working days.

Mouse Caspase 12 (CASP12) CLIA Kit

20-abx491877
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Please enquire.

Human Caspase 12 (CASP12) ELISA Kit

abx513694-96tests 96 tests
EUR 707
  • Shipped within 5-12 working days.

Mouse Casp12(Caspase-12) ELISA Kit

EM0436 96T
EUR 567.6
  • Detection range: 0.156-10 ng/ml
  • Uniprot ID: O08736
  • Alias: Casp12/CASP-12/Caspase-12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml

Rat Casp12(Caspase 12) ELISA Kit

ER0345 96T
EUR 567.6
  • Detection range: 0.156-10 ng/ml
  • Uniprot ID: Q920D5
  • Alias: Casp12/CASP-12/Caspase-12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.094 ng/ml

Mouse Caspase 12 (CASP12) ELISA Kit

SEA682Mu-10x96wellstestplate 10x96-wells test plate
EUR 4862.4
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Caspase 12 (CASP12) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Caspase 12 (CASP12) in tissue homogenates, cell lysates and other biological fluids.

Mouse Caspase 12 (CASP12) ELISA Kit

SEA682Mu-1x48wellstestplate 1x48-wells test plate
EUR 488.08
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Caspase 12 (CASP12) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Caspase 12 (CASP12) in tissue homogenates, cell lysates and other biological fluids.

Mouse Caspase 12 (CASP12) ELISA Kit

SEA682Mu-1x96wellstestplate 1x96-wells test plate
EUR 654.4
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Caspase 12 (CASP12) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Caspase 12 (CASP12) in tissue homogenates, cell lysates and other biological fluids.

Mouse Caspase 12 (CASP12) ELISA Kit

SEA682Mu-5x96wellstestplate 5x96-wells test plate
EUR 2644.8
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Caspase 12 (CASP12) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Caspase 12 (CASP12) in tissue homogenates, cell lysates and other biological fluids.

Mouse Caspase 12 (CASP12) ELISA Kit

4-SEA682Mu
  • EUR 4913.00
  • EUR 2595.00
  • EUR 655.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Caspase 12 elisa. Alternative names of the recognized antigen: CASP12P1
  • Cysteinyl Aspartate Specific Proteinases 12
  • Apoptosis-Related Cysteine Peptidase
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Caspase 12 (CASP12) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Caspase 12 ELISA Kit (CASP12)

RK02662 96 Tests
EUR 521

Caspase 12 (CASP12) Polyclonal Antibody (Mouse, Rat), APC-Cy7

4-PAA682Ra01-APC-Cy7
  • EUR 560.00
  • EUR 6286.00
  • EUR 1669.00
  • EUR 745.00
  • EUR 315.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: CASP12 (Met1~Thr244)
  • Buffer composition: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Description: A Rabbit polyclonal antibody against Mouse, Rat Caspase 12 (CASP12). This antibody is labeled with APC-Cy7.

CLIA kit for Rat CASP12 (Caspase 12)

E-CL-R0750 1 plate of 96 wells
EUR 584
  • Gentaur's CASP12 CLIA kit utilizes the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP12. Standards or samples are added to the micro CLIA plate wells and combined with the
  • Show more
Description: A sandwich CLIA kit for quantitative measurement of Rat CASP12 (Caspase 12) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Rat CASP12 (Caspase 12)

E-EL-R0165 1 plate of 96 wells
EUR 534
  • Gentaur's CASP12 ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP12. Standards or samples are added to the micro ELISA plate wells and combined with
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Rat CASP12 (Caspase 12) in samples from Serum, Plasma, Cell supernatant

Human CASP12/ Inactive caspase-12 ELISA Kit

E0355Hu 1 Kit
EUR 571

ELISA kit for Mouse CASP12 (Caspase 12)

ELK7681 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 12 (CASP12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 12 (CA
  • Show more
Description: A sandwich ELISA kit for detection of Caspase 12 from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Rabbit Anti Caspase-12 (Aa100-116) Polyclonal Antibody

CPBT-66303RC 0.1 mg
EUR 580

Casp12 ELISA Kit| Rat Caspase 12 ELISA Kit

EF017215 96 Tests
EUR 689

Casp12 ELISA Kit| Mouse Caspase-12 ELISA Kit

EF013086 96 Tests
EUR 689

Anti-Caspase-12 Antibody

A04700 100ug
EUR 471
Description: Rabbit Polyclonal Caspase-12 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

anti- Caspase 12 antibody

FNab01286 100µg
EUR 505.25
  • Immunogen: caspase 12(gene/pseudogene)
  • Uniprot ID: Q6UXS9
  • Research Area: Metabolism
Description: Antibody raised against Caspase 12

Anti-Caspase 12 antibody

PAab01286 100 ug
EUR 355

Caspase 12 (Caspase 12) Antibody

20-abx007974
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

Caspase 12 (Caspase 12) Antibody

abx432451-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

Caspase 12 (Caspase 12) Antibody

abx231286-100ug 100 ug
EUR 481
  • Shipped within 5-12 working days.

Polyclonal Caspase-12 Antibody

APR00058G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Caspase-12 . This antibody is tested and proven to work in the following applications:

Polyclonal Caspase-12 Antibody

APR06275G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Caspase-12 . This antibody is tested and proven to work in the following applications:

Polyclonal Caspase-12 Antibody

APR06276G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Caspase-12 . This antibody is tested and proven to work in the following applications:

Caspase 12 Polyclonal Antibody

ABP50853-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from the Internal region of human Caspase12
  • Applications tips:
Description: A polyclonal antibody for detection of Caspase 12 from Human. This Caspase 12 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Caspase12

Caspase 12 Polyclonal Antibody

ABP50853-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from the Internal region of human Caspase12
  • Applications tips:
Description: A polyclonal antibody for detection of Caspase 12 from Human. This Caspase 12 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Caspase12

Caspase 12 Polyclonal Antibody

ABP50853-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from the Internal region of human Caspase12
  • Applications tips:
Description: A polyclonal antibody for detection of Caspase 12 from Human. This Caspase 12 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Caspase12

Caspase-12 Rabbit pAb

A0217-100ul 100 ul
EUR 308

Caspase-12 Rabbit pAb

A0217-200ul 200 ul
EUR 459

Caspase-12 Rabbit pAb

A0217-20ul 20 ul
EUR 183

Caspase-12 Rabbit pAb

A0217-50ul 50 ul
EUR 223

Polyclonal Caspase-12 Antibody (Large)

APR06327G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Caspase-12 (Large). This antibody is tested and proven to work in the following applications:

Polyclonal Caspase-12 Antibody (Small)

APR06328G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Caspase-12 (Small). This antibody is tested and proven to work in the following applications:

Anti-CASP12 antibody

STJ112891 100 µl
EUR 277

Casp12 Polyclonal Antibody

A50097 100 µg
EUR 570.55
Description: fast delivery possible

Caspase-12 Antibody

24119-100ul 100ul
EUR 390

Caspase-12 Antibody

24120-100ul 100ul
EUR 390

Caspase 12 antibody

20R-1513 100 ug
EUR 673
Description: Rabbit polyclonal Caspase 12 antibody

Caspase 12 antibody

70R-11740 100 ug
EUR 460
Description: Rabbit polyclonal Caspase 12 antibody

Caspase-12 Antibody

3282-100
EUR 354

Caspase-12 Antibody

3282-30T
EUR 146

Caspase 12 antibody

70R-51166 100 ul
EUR 244
Description: Purified Polyclonal Caspase 12 antibody
hicstatistics
Utilizing genome-scale fashions to optimize nutrient present for sustained algal progress and lipid productiveness
  • Nutrient availability is crucial for progress of algae and totally different microbes used for producing worthwhile biochemical merchandise. Determining the optimum ranges of nutrient offers to cultures can eliminate feeding of additional nutritional vitamins, decreasing manufacturing costs and reducing nutrient air air pollution into the environment.

 

  • With the looks of omics and bioinformatics methods, it is now attainable to assemble genome-scale fashions that exactly describe the metabolism of microorganisms. On this study, a genome-scale model of the inexperienced alga Chlorella vulgaris (iCZ946) was utilized to predict feeding of a lot of nutritional vitamins, along with nitrate and glucose, beneath every autotrophic and heterotrophic conditions.

 

  • The goal carry out was modified from optimizing progress to instead minimizing nitrate and glucose uptake prices, enabling predictions of feed prices for these nutritional vitamins. The metabolic model administration (MMC) algorithm was validated for autotrophic progress, saving 18% nitrate whereas sustaining algal progress.

 

  • Furthermore, we obtained comparable progress profiles by concurrently controlling glucose and nitrate offers beneath heterotrophic conditions for every extreme and low ranges of glucose and nitrate. Lastly, the nitrate present was managed in an effort to retain protein and chlorophyll synthesis, albeit at a lower charge, beneath nitrogen-limiting conditions.

 

  • This model-driven cultivation method doubled all the volumetric yield of biomass, elevated fatty acid methyl ester (FAME) yield by 61%, and enhanced lutein yield virtually Three fold compared with nitrogen starvation. This study introduces a administration methodology that integrates omics info and genome-scale fashions in an effort to optimize nutrient offers based mostly totally on the metabolic state of algal cells in a number of nutrient environments.

 

  • This technique might rework bioprocessing administration proper right into a strategies biology-based paradigm applicable for quite a lot of species in an effort to limit nutrient inputs, in the reduction of processing costs, and optimize biomanufacturing for the next expertise of fascinating biotechnology merchandise.

 

Melting dsDNA Donor Molecules Vastly Improves Precision Genome Enhancing in Caenorhabditiselegans

 

CRISPR genome modifying has revolutionized genetics in numerous organisms. Inside the nematode Caenorhabditiselegans one injection into each of the two gonad arms of an grownup hermaphrodite exposes tons of of meiotic germ cells to modifying mixtures, permitting the restoration of a lot of indels or small precision edits from each effectively injected animal. Sadly, considerably for prolonged insertions, modifying efficiencies can differ broadly, necessitating a lot of injections, and generally requiring co-selection strategies.

 

Proper right here we current that melting double stranded DNA (dsDNA) donor molecules earlier to injection will improve the frequency of tangible homology-directed restore (HDR) by a lot of fold for longer edits. We describe troubleshooting strategies that permit persistently extreme modifying efficiencies ensuing, as an illustration, in as a lot as 100 unbiased GFP knock-ins from a single injected animal. These efficiencies make C. elegans by far the best metazoan to genome edit, eradicating limitations to the use and adoption of this facile system as a model for understanding animal biology.

 

Longitudinal Assertion of Muscle Mass over 10 Years Consistent with Serum Calcium Ranges and Calcium Consumption amongst Korean Adults Aged 50 and Older: The Korean Genome and Epidemiology Analysis

 

The intention of this study was to analysis the longitudinal change in muscle mass over 10 years in line with serum calcium ranges and calcium consumption. An entire of 1497 males and 1845 women aged 50 years and older had been included. Very important muscle loss (SML) was outlined as a 5% or bigger loss from baseline, whereas time-dependent progress of SML was assessed in line with quartiles for corrected calcium stage and every day calcium consumption using Cox regression fashions.

 

The incidence of SML was 6.7 and 7.7 per 100-person-years amongst men and women, respectively. Groups with the underside corrected calcium ranges had additional excellent SML than these with elevated calcium ranges, irrespective of intercourse. The connection between SML and calcium consumption was very important solely amongst women. The hazard ratio for SML per 1 mmol/L improve in corrected calcium stage was 0.236 and 0.237 for men and women, respectively. In conclusion, low serum calcium ranges may predict SML amongst adults aged ≥ 50 years, whereas low calcium consumption is also a predictor for muscle loss amongst women. Subsequently, encouraging dietary calcium consumption amongst middle-aged and older adults for preservation of muscle mass must be thought-about.

hicstatistics
hicstatistics

Viral Bcl2s‘ transmembrane domain interact with host Bcl2 proteins to control cellular apoptosis

Viral control of programmed cell death relies in part on the expression of viral analogs of the B-cell lymphoma 2 (Bcl2) protein known as viral Bcl2s (vBcl2s). vBcl2s control apoptosis by interacting with host pro- and anti-apoptotic members of the Bcl2 family.
Here, we show that the carboxyl-terminal hydrophobic region of herpesviral and poxviral vBcl2s can operate as transmembrane domains (TMDs) and participate in their homo-oligomerization. Additionally, we show that the viral TMDs mediate interactions with cellular pro- and anti-apoptotic Bcl2 TMDs within the membrane.
Furthermore, these intra-membrane interactions among viral and cellular proteins are necessary to control cell death upon an apoptotic stimulus. Therefore, their inhibition represents a new potential therapy against viral infections, which are characterized by short- and long-term deregulation of programmed cell death.
miRNAs play an important role in the pathogenesis of intervertebral disc degeneration (IDD). The role and the underlying mechanism of miR-424-5p in human nucleus pulposus (NP) are still unknown. We aimed to explore the role of miR-424-5p in IDD. Real-time PCR was used to detect the expression of miR-424-5p and Bcl2 in IDD tissues and idiopathic scoliosis tissues.
Human NP cells were used in our study. MTT and Hoechst apoptosis assays were used to detect the proliferation and apoptosis of NP cells, respectively. Western blotting assays were used to detect the expression levels of Bcl-2, cleaved caspase-3, cleaved caspase-9, caspase-3 and caspase-9 in degenerative NP cells.
A luciferase reporter assay was applied to confirm the relationship between miR-424-5p and Bcl2. Our results showed that the expression of miR-424-5p was increased and Bcl2 was decreased in degenerative NP cells. miR-425-5p expression was negatively correlated with Bcl2 expression in IDD tissues.
Suppression of miR-424-5p using an inhibitor increased Bcl2 expression at both the mRNA and protein levels, and it promoted cell viability and inhibited apoptosis. Furthermore, the levels of cleaved caspase-3 and cleaved caspase-9 were downregulated in miR-424-5p-silenced NP cells. Interestingly, we found that silencing miR-424-5p increased p62 expression at both the mRNA and protein levels. Finally, a luciferase reporter assay verified the binding of the miR-424-5p and the 3’UTR of Bcl2. These results suggested that silencing miR-424-5p suppressed NP cell apoptosis by upregulating Bcl2. Therefore, miR-424-5p might be a novel target for IDD therapies.

 

Xpert Total Histone Extraction Kit
H7200-050 50 ext
EUR 361
Xpert Total Histone Extraction Kit
H7200-100 100 ext
EUR 509
EpiQuik Total Histone H3 Quantification Kit (Colorimetric)
P-3062 96 Assays
EUR 759.05
Description: Ask the seller for details
EpiQuik Total Histone H3 Quantification Kit (Fluorometric)
P-3063 96 Assays
EUR 788.05
Description: The best epigenetics products
EpiQuik Total Histone H4 Quantification Kit (Colorimetric)
P-3072 96 Assays
EUR 759.05
Description: reagents widely cited
EpiQuik Total Histone H4 Quantification Kit (Fluorometric)
P-3073 96 Assays
EUR 759.05
Description: Ask the seller for details
EpiQuik Nuclear Extraction Kit
OP-0002 100 Assays
EUR 292.15
Description: kits suitable for this type of research
EpiQuik Circulating Total Histone H3 Quantification Kit (Colorimetric)
P-3091 96 Assays
EUR 773.55
Description: kits suitable for this type of research
EpiQuik Whole Cell Extraction Kit
OP-0003 100 Extractions
EUR 270.4
Description: Ask the seller for details
EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric)
P-4030 96 Assays
EUR 773.55
Description: reagents widely cited
EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Fluorometric)
P-4031 96 Assays
EUR 788.05
Description: Ask the seller for details
EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Colorimetric)
P-4032 96 Assays
EUR 788.05
Description: The best epigenetics products
EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Fluorometric)
P-4033 96 Assays
EUR 788.05
Description: kits suitable for this type of research
Total RNA Extraction Kit
K2014005 1 kit
EUR 344
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein Extraction Kit
K3011010 1 kit
EUR 370
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein Extraction Kit
BSP003 50Preps
EUR 109.16
  • Product category: Molecular Biology Kits/Protein - Extraction/Animal
Mammalian Total Protein Extraction Kit
20-abx098853
  • EUR 356.00
  • EUR 105.00
  • 100 ml
  • 2 ml
  • Shipped within 5-10 working days.
AnaPrep Total RNA Extraction Kit
Z1322015 1 kit (48 extractions) Including all required plastic disposables
EUR 308
Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications.
Human Total PSA (t-PSA) ELISA Kit
PRB-5049-TOTAL 96 assays
EUR 572
Total Protein Extraction Kit: TM Buffer
K3011010-1 13 ml
EUR 162
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein Extraction Kit: 50X PI
K3011010-2 260 ul
EUR 289
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Rapid Animal Total RNA Extraction Kit
AT4181 50Preps
EUR 88.06
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal
Rapid Animal Total RNA Extraction Kit
AT4182 250preps
EUR 202.25
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal
EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free)
OP-0022 100 Extractions
EUR 292.15
Description: kits suitable for this type of research
AffiSelect Total Protein Extraction Solution
A0710-015 15X1ml
EUR 142
Bacterial Total Protein Extraction Reagent
abx090632-50100assays 50-100 assays
EUR 258
  • Shipped within 5-10 working days.
Plant Total Protein Extraction Reagent
abx090633-50100assays 50-100 assays
EUR 230
  • Shipped within 5-10 working days.
EpiQuik Histone H3 Citrullination ELISA Kit
P-3095 96 Assays
EUR 947.55
Description: reagents widely cited
EpiQuik Methyl-Histone H3K4 ChIP Kit
P-2007 48 Reactions
EUR 860.55
Description: fast delivery possible
EpiQuik Acetyl-Histone H3 ChIP Kit
P-2010 48 Reactions
EUR 875.05
Description: The best epigenetics products
EpiQuik Acetyl-Histone H4 ChIP Kit
P-2011 48 Reactions
EUR 875.05
Description: kits suitable for this type of research
EpiQuik Methyl-Histone H3K27 ChIP Kit
P-2015 48 Reactions
EUR 889.55
Description: The best epigenetics products
3-min Bacterial Total Protein Extraction Kit
P512L NULL
EUR 0
Human Total PSA (t-PSA) ELISA Kit
PRB-5049-TOTAL-5 5 x 96 assays
EUR 2283
RNA isolater Total RNA Extraction Reagent
R401-01 100 ml
EUR 133
EpiQuik Global Histone H3 Acetylation Assay Kit
P-4008 96 Assays
EUR 788.05
Description: kits suitable for this type of research
EpiQuik Global Histone H4 Acetylation Assay Kit
P-4009 96 Assays
EUR 860.55
Description: fast delivery possible
EpiQuik Tri-Methyl-Histone H3K9 ChIP Kit
P-2006 48 Reactions
EUR 875.05
Description: kits suitable for this type of research
EpiQuik Tissue Methyl-Histone H3K9 ChIP Kit
P-2008 48 Reactions
EUR 875.05
Description: reagents widely cited
EpiQuik Tissue Methyl-Histone H3K4 ChIP Kit
P-2009 48 Reactions
EUR 875.05
Description: Ask the seller for details
EpiQuik Tissue Acetyl-Histone H3 ChIP Kit
P-2012 48 Reactions
EUR 875.05
Description: fast delivery possible
EpiQuik Tissue Acetyl-Histone H4 ChIP Kit
P-2013 48 Reactions
EUR 875.05
Description: reagents widely cited
EpiQuik Tissue Methyl-Histone H3K27 ChIP Kit
P-2016 48 Reactions
EUR 889.55
Description: kits suitable for this type of research
EpiQuik Global Histone H3K4 Methylation Assay Kit
P-3017 96 Assays
EUR 889.55
Description: reagents widely cited
EpiQuik Global Histone H3K9 Methylation Assay Kit
P-3018 96 Assays
EUR 889.55
Description: Ask the seller for details
3-min Total Protein Extraction Kit (Animal cells)
P501 NULL
EUR 0