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Zinc(II) niflumato complex effects on MMP activity and gene expression in human endometrial cell lines
Endometriosis is a persistent inflammatory illness which more and more impacts younger girls underneath 35 years of age and results in subfertility even infertility. Evaluation of the cytotoxic impact of zinc(II) niflumato advanced with neocuproine ([Zn(neo)(nif)2] or Zn-Nif) on immortalized human endometriotic cell line (12Z) and on management immortalized human endometrial stromal cell line (hTERT) was carried out utilizing xCELLigence know-how for roughly 72 h following the remedy with Zn-Nif in addition to cell viability Trypan Blue Assay. 12Z cell line proliferated extra slowly in comparison with unaffected cells, whereas hTERT cells didn’t present related conduct after remedy.
The advanced in all probability reduces the impact of pro-inflammatory pathways as a result of impact of NSAID, whereas presence of zinc may scale back the extent of ROS and regulate ER2 ranges and MMP exercise. The noticed results and excessive selectivity for quickly proliferating cells with elevated inflammatory exercise recommend an excellent prognosis of profitable lower of endometriosis stage with this advanced.

Mineralized polyplexes for gene supply: Enchancment of transfection effectivity as a consequence of calcium incubation and never mineralization

Gene remedy is an rising discipline wherein nucleic acids are used to manage protein expression. The need of delivering nucleic acids to particular cell varieties and intracellular websites calls for the usage of extremely specialised gene carriers. As a provider modification method, mineralization has been efficiently used to change viral and non-viral carriers, offering new properties that in the end intention to extend the transfection effectivity. Nonetheless, for the particular case of polyplexes utilized in gene remedy, latest literature exhibits that interplay with calcium, a elementary step of mineralization, could be efficient to extend transfection effectivity, leaving an ambiguity about of the function of mineralization for this sort of gene carriers.
To reply this query and to disclose the properties chargeable for rising transfection effectivity, we mineralized poly(aspartic acid) coated polyplexes at various CaCl2 and Na3PO4 concentrations, and evaluated the resultant carriers for physicochemical and morphological traits, in addition to transfection and supply effectivity with MC3T3-E1 mouse osteoblastic cells.
We discovered that each mineralization and calcium incubation positively affected the transfection effectivity and uptake of polyplexes in MC3T3-E1 cells. Nonetheless, this impact originated from the properties achieved by polyplexes after the calcium incubation step which might be maintained after mineralization, together with particle measurement enhance, improved pDNA binding, and adjustment of zeta potential. Contemplating that mineralization generally is a longer course of than calcium incubation, we discover that calcium incubation could be enough and most popular if improved transfection effectivity in vitro is the one impact desired.
Staphylococcus epidermidis has been not too long ago acknowledged as an rising nosocomial pathogen. There are considerations over the rising virulence potential of this commensal as a result of capabilities of transferring cell genetic parts to Staphylococcus aureus by way of staphylococcal chromosomal cassette (SCCmec) and the carefully associated arginine catabolic cell factor (ACME) and the copper and mercury resistance island (COMER). The potential pathogenicity of S. epidermidis, significantly from blood stream infections, has been poorly investigated. On this research, 24 S. epidermidis remoted from blood stream infections from Oman had been investigated utilizing entire genome sequence evaluation. Core genome phylogenetic bushes revealed one third of the isolates belong to the multidrug resistance ST-2.
Genomic evaluation unraveled a typical incidence of SCCmec sort IV and ACME factor predominantly sort I organized in a composite island. The genetic composition of ACME was extremely variable amongst isolates of identical or totally different STs. The COMER-like island was absent in all of our isolates. Lowered copper susceptibility was noticed amongst isolates of ST-2 and ACME sort I, adopted by ACME sort V. In conclusion, on this work, we establish a prevalent incidence of extremely variable ACME parts in several hospital STs of S. epidermidis in Oman, thus strongly suggesting the speculation that ACME varieties developed from carefully associated STs.
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Antibiotics, Multidrug-Resistant Micro organism, and Antibiotic Resistance Genes: Indicators of Contamination in Mangroves?

Multidrug-resistant micro organism and antibiotic resistance genes will be monitored as indicators of contamination in a number of environments. Mangroves are among the many most efficient ecosystems, and though they are often resilient to the motion of local weather phenomena, their equilibrium will be affected by anthropogenic actions. Relating to the presence and persistence of multidrug-resistant micro organism in mangroves, it is not uncommon to suppose that this ecosystem can operate as a reservoir, which might disperse the antibiotic resistance capability to human pathogens, or function a filter to remove drug-resistant genes.
The attainable affect of anthropogenic actions carried out close to mangroves is reviewed, together with wastewater remedy, meals manufacturing techniques, leisure, and tourism. Hostile results of antibiotic resistance genes or multidrug-resistant micro organism, thought-about as rising contaminants, haven’t been reported but in mangroves. Quite the opposite, mangrove ecosystems generally is a pure technique to remove antibiotics, antibiotic-resistant micro organism, and even antibiotic-resistant genes from the surroundings.
Though mangroves’ function in reducing antibiotics and antibiotic resistance genes from the surroundings is being proposed, the mechanisms by which these vegetation scale back these rising contaminants haven’t been elucidated and want additional research. Moreover, additional analysis is required on the consequences of antibiotics and antibiotic-resistant micro organism in mangroves to generate an evaluation of the human contribution to the degradation of this particular ecosystem in addition to to outline if these contaminants can be utilized as indicators of contamination in mangrove ecosystems.
Irrigation water is effectively generally known as potential supply of pathogens in recent produce. Nonetheless, its function in transferring antibiotic resistance determinants is much less effectively investigated. Due to this fact, we analyzed the contribution of floor and faucet water to the resistome of overhead-irrigated chive vegetation. Area-grown chive was irrigated with both floor water (R-system) or faucet water (D-system), from planting to reap. Water alongside the 2 irrigation chains in addition to the respective vegetation had been repeatedly sampled and screened for 264 antibiotic resistance genes (ARGs) and cell genetic parts (MGEs), utilizing high-capacity qPCR.
Differentially plentiful (DA) ARGs had been decided by evaluating the 2 systems. On R-chive, β-lactam ARGs, multidrug-resistance (MDR) determinants, and MGEs had been most plentiful, whereas D-chive featured DA ARGs from the vancomycin class. Variety and variety of DA ARGs was the best on younger chives, strongly diminished at harvest, and elevated once more on the finish of shelf life.
Most ARGs extremely enriched on R- in comparison with D-chive had been additionally enriched in R- in comparison with D-sprinkler water, indicating that water performed a significant function in ARG enrichment. Of be aware, blaKPC was detected at excessive ranges in floor water and chive. We conclude that water high quality considerably impacts the resistome of the irrigated produce.
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Genetic Aberrations of DNA Repair Pathways in Prostate Cancer: Translation to the Clinic
Prostate most cancers (PC) is the second most typical most cancers in males worldwide. Because of the large-scale sequencing efforts, there may be presently a greater understanding of the genomic panorama of PC. The identification of defects in DNA restore genes has led to medical research that present a powerful rationale for growing poly (ADP-ribose) polymerase (PARP) inhibitors and DNA-damaging brokers on this molecularly outlined subset of sufferers.
The identification of molecularly outlined subgroups of sufferers has additionally different medical implications; for instance, we now know that carriers of breast most cancers 2 (BRCA2) pathogenic sequence variants (PSVs) have elevated ranges of serum prostate particular antigen (PSA) at analysis, elevated proportion of excessive Gleason tumors, elevated charges of nodal and distant metastases, and excessive recurrence fee; BRCA2 PSVs confer decrease general survival (OS). Distinct tumor PSV, methylation, and expression patterns have been recognized in BRCA2 in contrast with non-BRCA2 mutant prostate tumors.
A number of DNA injury response and restore (DDR)-targeting brokers are presently being evaluated both as single brokers or together in sufferers with PC. On this overview article, we spotlight the biology and medical implications of deleterious inherited or acquired DNA restore pathway aberrations in PC and provide an outline of recent brokers being developed for the remedy of PC.

Analyzing Plant Gene Focusing on Outcomes and Conversion Tracts with Nanopore Sequencing

The high-throughput molecular evaluation of gene concentrating on (GT) occasions is made technically difficult by the residual presetabce of donor molecules. Giant donor molecules prohibit primer placement, leading to lengthy amplicons that can’t be readily analyzed utilizing customary NGS pipelines or qPCR-based approaches resembling ddPCR. In crops, removing of extra donor is time and useful resource intensive, typically requiring plant regeneration and weeks to months of effort.
Right here, we utilized Oxford Nanopore Amplicon Sequencing (ONAS) to bypass the constraints imposed by donor molecules with 1 kb of homology to the goal and dissected GT outcomes at three loci in Nicotiana benthamia leaves. We developed a novel bioinformatic pipeline, Phased ANalysis of Genome Modifying Amplicons (PANGEA), to cut back the impact of ONAS error on amplicon evaluation and captured tens of 1000’s of somatic plant GT occasions.
Moreover, PANGEA allowed us to gather 1000’s of GT conversion tracts 5 days after reagent supply with no choice, revealing that almost all occasions utilized tracts lower than 100 bp in size when incorporating an 18 bp or three bp insertion. These knowledge reveal the usefulness of ONAS and PANGEA for plant GT evaluation and supply a mechanistic foundation for future plant GT optimization.

Genome Sequence Evaluation of the Fungal Pathogen Fusarium graminearum Utilizing Oxford Nanopore Know-how

Fusarium graminearum is a plant pathogen of world significance which causes not solely important yield loss but additionally crop spoilage as a consequence of mycotoxins that render grain unsafe for human or livestock consumption. Though the complete genome of a number of F. graminearum isolates from completely different elements of the world have been sequenced, there aren’t any comparable research of isolates originating from China.
The present research sought to deal with this by sequencing the F. graminearum isolate FG-12, which was remoted from the roots of maize seedlings exhibiting typical signs of blight rising within the Gansu province, China, utilizing Oxford Nanopore Know-how (ONT). The FG-12 isolate was discovered to have a 35.9 Mb genome comprised of 5 scaffolds comparable to the 4 chromosomes and mitochondrial DNA of the F. graminearum sort pressure, PH-1.
The genome was discovered to comprise an roughly 2.23% repetitive sequence and encode 12,470 predicted genes. Extra bioinformatic evaluation recognized 437 genes that had been predicted to be secreted effectors, certainly one of which was confirmed to set off a hypersensitive responses (HR) within the leaves of Nicotiana benthamiana throughout transient expression experiments using agro-infiltration. The F. graminearum FG-12 genome sequence and annotation knowledge produced within the present research present a particularly helpful useful resource for each intra- and inter-species comparative analyses in addition to for gene purposeful research, and will enormously advance our understanding of this essential plant pathogen.
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Prevalence of Virulence Genes and Antimicrobial Resistance of E. coli O157:H7 Remoted from the Beef Carcass of Bahir Dar Metropolis, Ethiopia

Ecoli O157:H7 is among the most virulent foodborne pathogens. The purpose of this research was to isolate E. coli O157:H7, decide virulence genes carried by the organism, and assess the antimicrobial susceptibility sample of the isolates from beef carcass samples at Bahir Dar metropolis. Swab samples (n = 280) had been collected from the carcass of cattle slaughtered on the abattoir and processed utilizing sorbitol MacConkey agar supplemented with cefixime telluride and confirmed with latex agglutination take a look at.
A polymerase chain response was carried out on isolates for the detection of virulence genes stx1stx2hlyA, and eae. Antimicrobial susceptibility testing was carried out utilizing the disk diffusion technique. Of 280 samples processed, 25 (8.9%) isolates had been optimistic. Out of 25 isolates subjected for molecular detection, 8 (32%) and 14 (56%) isolates possessed stx1 and stx2 genes, respectively; from these, 5 (20%) isolates had each genes for the manufacturing of Shiga toxins.
In contrast from different virulent genes comparatively increased proportion of 18 (72%) isolates carried the hlyA gene. Solely 5 (2%) isolates had been optimistic for eae. Resistance was detected in all 25 (100%) isolates and three (12%) towards clindamycin and trimethoprim, respectively. This research end result highlights the potential risk to public well being. The abattoir staff should be conscious concerning the pathogen and may comply with applicable practices to stop contamination of meat supposed for human consumption.

Genome-scale RNAi screens in African trypanosomes

Genome-scale genetic screens enable researchers to quickly establish the genes and proteins that influence a specific phenotype of curiosity. In African trypanosomes, RNA interference (RNAi) knockdown screens have revealed mechanisms underpinning drug resistance, drug transport, prodrug metabolism, quorum sensing, genome replication, and gene expression management.
RNAi screening has additionally been remarkably efficient at highlighting promising potential antitrypanosomal drug targets. The primary ever RNAi library display was applied in African trypanosomes, and genome-scale RNAi screens and different associated approaches proceed to have a serious influence on trypanosomatid analysis. Right here, I overview these impacts when it comes to each discovery and translation.
The latest software of macroecological instruments and ideas has made it potential to establish constant patterns within the distribution of microbial biodiversity, which enormously improved our understanding of the microbial world at massive scales. Nevertheless, the distribution of microbial capabilities stays largely uncharted from the macroecological perspective. Right here, we used macroecological fashions to look at how the genes encoding the purposeful capabilities of microorganisms are distributed inside and throughout soil methods.
Fashions constructed utilizing purposeful gene array knowledge from 818 soil microbial communities confirmed that the occupancy-frequency distributions of genes had been bimodal in each studied website, and that their rank-abundance distributions had been finest described by a lognormal mannequin. As well as, the relationships between gene occupancy and abundance had been optimistic in all websites.
This allowed us to establish genes with excessive abundance and ubiquitous distribution (core) and genes with low abundance and restricted spatial distribution (satellites), and to indicate that they encode completely different units of microbial traits. Widespread genes encode microbial traits associated to the primary biogeochemical cycles (C, N, P and S) whereas uncommon genes encode traits associated to adaptation to environmental stresses, resembling nutrient limitation, resistance to heavy metals and degradation of xenobiotics.
Total, this research characterised for the primary time the distribution of microbial purposeful genes inside soil methods, and spotlight the curiosity of macroecological fashions for understanding the purposeful group of microbial methods throughout spatial scales.
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The impact of anionic polymers on gene delivery: how composition and assembly help evading the toxicity-efficiency dilemma
Cationic polymers have been broadly studied for non-viral gene supply on account of their means to bind genetic materials and to work together with mobile membranes. Nonetheless, their charged nature carries the danger of elevated cytotoxicity and interplay with serum proteins, limiting their potential in vivo utility. Due to this fact, hydrophilic or anionic shielding polymers are utilized to counteract these results. Herein, a sequence of micelle-forming and micelle-shielding polymers have been synthesized through RAFT polymerization.
The copolymer poly[(n-butyl acrylate)-b-(2-(dimethyl amino)ethyl acrylamide)] (P(nBA-b-DMAEAm)) was assembled into cationic micelles and totally different shielding polymers have been utilized, i.e., poly(acrylic acid) (PAA), poly(4-acryloyl morpholine) (PNAM) or P(NAM-b-AA) block copolymer. These techniques have been in comparison with a triblock terpolymer micelle comprising PAA as the center block. The assemblies have been investigated relating to their morphology, interplay with pDNA, cytotoxicity, transfection effectivity, polyplex uptake and endosomal escape.
The bare cationic micelle exhibited superior transfection effectivity, however elevated cytotoxicity. The addition of protecting polymers led to diminished toxicity. Particularly, the triblock terpolymer micelle satisfied with excessive cell viability and no important loss in effectivity. The very best shielding impact was achieved by layering micelles with P(NAM-b-AA) supporting the colloidal stability at impartial zeta potential and utterly restoring cell viability whereas sustaining reasonable transfection efficiencies. The excessive potential of this micelle-layer-combination for gene supply was illustrated for the primary time.

Genetic Evaluation, Inhabitants Construction, and Characterisation of Multidrug-Resistant Klebsiella pneumoniae from the Al-Hofuf Area of Saudi Arabia

Multidrug-resistant Klebsiella pneumoniae (MDR-KP) is a significant public well being drawback that’s globally related to illness outbreaks and excessive mortality charges. Because the world seeks options to such pathogens, international and regional surveillance is required. The goal of the current research was to look at the antimicrobial susceptibility sample and clonal relatedness of Klebsiella pneumoniae isolates collected for a interval of three years by way of pulse discipline gel electrophoresis (PFGE).
Isolate IDs, antimicrobial assays, ESBL-production, and minimal inhibitory concentrations (MICs) have been examined with the Vitek 2 Compact Automated System. IDs have been confirmed by 16S rRNA gene sequencing, with the ensuing sequences being deposited in NCBI databases. DNA was extracted and resistance genes have been detected by PCR amplification with applicable primers. Isolates have been intensive (31%) and multidrug-resistant (65%).
Pulsotype clusters grouped the isolates into 22 band profiles that confirmed no particular sample with phenotypes. Of the isolates, 98% have been ESBL-KP, 69% have been carbapenem-resistant Enterobacteriaceae (CRE) strains, and 72.5% comprised the carriage of two MBLs (SIM and IMP). Integrons (ISAba1, ISAba2, and IS18) have been detected in 69% of the MDR-KP. Moreover, OXA-23 was detected in 67% of the isolates. This research subsequently demonstrates clonal range amongst scientific Ok. pneumoniae, confirming that this bacterium has entry to an infinite pool of genes that confer excessive resistance-developing potential.

Full Genome Sequencing of Leptospira interrogans Isolates from Malaysia Reveals Large Genome Rearrangement however Excessive Conservation of Virulence-Related Genes

The power of Leptospirae to persist in environments and animal hosts however to trigger clinically extremely variable illness in people has made leptospirosis the most typical zoonotic illness. Contemplating the paucity of knowledge on variation in full genomes of human pathogenic Leptospirae, we’ve used a mix of Single Molecule Actual-Time (SMRT) and Illumina sequencing to acquire full genome sequences of six human scientific L. interrogans isolates from Malaysia.
  • All six contained the bigger (4.28-4.56 Mb) and smaller (0.34-0.395 Mb) chromosome typical of human pathogenic Leptospirae and 0-7 plasmids. Solely 24% of the plasmid sequences could possibly be matched to databases. We recognized a chromosomal core genome of 3318 coding sequences and strain-specific accent genomes of 49-179 coding sequences.
  • These sequences enabled detailed genomic pressure typing (Genome BLAST Distance Phylogeny, DNA-DNA hybridization, and multi locus sequence typing) and phylogenetic classification (whole-genome SNP genotyping). Regardless that there was some shared synteny and collinearity throughout the six genomes, there was proof of main genome rearrangement, doubtless pushed by horizontal gene switch and homologous recombination.
  • Cellular genetic parts have been recognized in all strains in extremely various numbers, together with within the rfb locus, which defines serogroups and contributes to immune escape and pathogenesis. Then again, there was excessive conservation of virulence-associated genes together with these referring to sialic acid, alginate, and lipid A biosynthesis.
  • These findings counsel (i) that the antigenic variation, adaption to numerous host environments, and broad spectrum of virulence of L. interrogans are partially on account of a excessive diploma of genomic plasticity and (ii) that human pathogenic strains keep a core set of genes required for virulence.
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Genomic Analyses of Penicillium Species Have Revealed Patulin and Citrinin Gene Clusters and Novel Loci Concerned in Oxylipin Manufacturing

Blue mildew of apple is attributable to a number of totally different Penicillium species, amongst which P. expansum and P. solitum are essentially the most ceaselessly remoted. P. expansum is essentially the most aggressive species, and P. solitum could be very weak when infecting apple fruit throughout storage. On this research, we report full genomic analyses of three totally different Penicillium species: P. expansum R21 and P. crustosum NJ1, remoted from saved apple fruit; and Pmaximae 113, remoted in 2013 from a flooded house in New Jersey, USA, within the aftermath of Hurricane Sandy. Patulin and citrinin gene cluster analyses defined the shortage of patulin manufacturing in NJ1 in comparison with R21 and lack of citrinin manufacturing in all three strains.
Drosophila bioassay demonstrated that volatiles emitted by Psolitum SA and Ppolonicum RS1 have been extra poisonous than these from Pexpansum and P. crustosum strains (R27, R11, R21, G10, and R19). The toxicity was hypothesized to be associated to manufacturing of eight-carbon oxylipins. Putative lipoxygenase genes have been recognized in Pexpansum and Pmaximae strains, however not in Pcrustosum. Our information will present a greater understanding of Penicillium spp. complicated secondary metabolic capabilities, particularly regarding the genetic bases of mycotoxins and poisonous VOCs.
Pig-to-human xenotransplantation appears to be the response to the up to date scarcity of tissue/organ donors. Sadly, the phylogenetic distance between pig and human implies hyperacute xenograft rejection. On this research, we examined the speculation that combining expression of human α1,2-fucosyltransferase (hFUT2) and α-galactosidase A (hGLA) genes would enable for removing of this impediment in porcine transgenic epidermal keratinocytes (PEKs). We sought to find out not solely the expression profiles of recombinant human α1,2-fucosyltransferase (rhα1,2-FT) and α-galactosidase A (rhα-Gal A) proteins, but in addition the relative abundance (RA) of Galα1→3Gal epitopes within the PEKs stemming from not solely hFUT2 or hGLA single-transgenic and hFUT2×hGLA double-transgenic pigs. Our confocal microscopy and Western blotting analyses revealed that each rhα1,2-FT and rhα-Gal A enzymes have been overabundantly expressed in respective transgenic PEK traces.
Furthermore, the semiquantitative ranges of Galα1→3Gal epitope that have been assessed by lectin fluorescence and lectin blotting have been discovered to be considerably diminished in every variant of genetically modified PEK line as in comparison with these noticed within the management nontransgenic PEKs. Notably, the bi-transgenic PEKs have been characterised by considerably lessened (however nonetheless detectable) RAs of Galα1→3Gal epitopes as in comparison with these recognized for each varieties of mono-transgenic PEK traces. Moreover, our present investigation confirmed that the coexpression of two protecting transgenes gave rise to enhanced abrogation of Galα→3Gal epitopes in hFUT2×hGLA double-transgenic PEKs.
To summarize, detailed estimation of semiquantitative profiles for human α-1,2-FT and α-Gal A proteins adopted by identification of the extent of abrogating the abundance of Galα1→3Gal epitopes within the ex vivo expanded PEKs stemming from mono- and bi-transgenic pigs have been discovered to be a sine qua non situation for effectively ex situ defending steady traces of skin-derived somatic cells inevitable in additional research.
The latter is because of be targeted on figuring out epigenomic reprogrammability of single- or double-transgenic cell nuclei inherited from grownup cutaneous keratinocytes in porcine nuclear-transferred oocytes and corresponding cloned embryos. To our data, this idea was proven to signify a very new strategy designed to generate and multiply genetically reworked pigs by somatic cell cloning for the wants of reconstructive drugs and dermoplasty-mediated tissue engineering of human integumentary system.
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The Genetic Diversification of a Single Bluetongue Virus Strain Using an In Vitro Model of Alternating-Host Transmission

Bluetongue virus (BTV) is an arbovirus that has been related to dramatic epizootics in each wild and residential ruminants in latest a really very long time. As a segmented, double-stranded RNA virus, BTV can evolve by a number of mechanisms because of its genomic constructing. Nonetheless, the have an effect on of BTV’s alternating-host transmission cycle on the virus’s genetic diversification stays poorly understood. Full genome sequencing approaches present a platform for investigating the have an effect on of host-alternation all by all ten segments of BTV’s genome.

 

To grasp the function of alternating hosts in BTV’s genetic diversification, a subject isolate was passaged beneath three totally completely completely different situations: (i) serial passages in Culicoidessonorensis cells, (ii) serial passages in bovine pulmonary artery endothelial cells, or (iii) alternating passages between insect and bovine cells.

 

Aliquots of virus had been sequenced, and single nucleotide variants had been acknowledged. Measures of viral inhabitants genetics had been used to quantify the genetic diversification that occurred. Two consensus variants in segments 5 and 10 occurred in virus from all three situations.

 

Whereas variants arose all by all passages, measures of genetic differ remained largely comparable all by cell customized situations. Irrespective of passage in a relaxed in vitro system, we discovered that this BTV isolate exhibited genetic stability all by passages and situations. Our findings underscore the expensive function that full genome sequencing could play in enhancing understanding of viral evolution and spotlight the genetic stability of BTV.

Novel Gene Rearrangement and the Full Mitochondrial Genome of Cynoglossusmonopus: Insights into the Envolution of the Household Cynoglossidae (Pleuronectiformes)

 

Cynoglossusmonopus, a small benthic fish, belongs to the Cynoglossidae, Pleuronectiformes. It was not normally studied because of its low abundance and cryptical life-style. With the intention to know the mitochondrial genome and the phylogeny in Cynoglossidae, all of the mitogenome of C. monopus has been sequenced and analyzed for the primary time. The entire dimension is 16,425 bp, usually containing 37 genes with novel gene rearrangements. The tRNA-Gln gene is inverted from the sunshine to the heavy strand and translocated from the downstream of tRNA-Ile gene to its upstream. The administration area (CR) translocated downstream to the three’-end of ND1 gene adjoining to inverted to tRNA-Gln and left a 24 bp hint fragment inside the real place.

 

 

The phylogenetic timber had been reconstructed by Bayesian inference (BI) and most likelihood (ML) strategies based completely on the mitogenomic data of 32 tonguefish species and two outgroups. The outcomes help the concept Cynoglossidae is a monophyletic group and degree out that C. monopus has the closest phylogenetic relationship with C. puncticeps.

 

By combining fossil data and mitogenome data, the time-calibrated evolutionary tree of households Cynoglossidae and Soleidae was firstly supplied, and it was indicated that Cynoglossidae and Soleidae had been differentiated from one another all by Paleogene, and the evolutionary technique of household Cynoglossidae coated the Quaternary, Neogene and Paleogene durations.

 

Genome-Broad Identification and Characterization of the SHI-Associated Sequence Gene Household in Rice

 

Rice (Oryzasativa) yield is correlated to varied elements. Transcription regulators are necessary elements, akin to the frequently SHORT INTERNODES-related sequences (SRSs), which encode proteins with single zinc finger motifs. Nonetheless, data relating to the evolutionary and smart traits of the SRS gene household members in rice is inadequate.

 

Subsequently, we carried out a genome-wide screening and characterization of the OsSRS gene household in Oryzasativa japonica rice. We furthermore examined the SRS proteins from 11 rice sub-species, consisting of three cultivars, 6 wild varieties, and some completely completely different genome sorts. SRS members from maize, sorghum, Brachypodiumdistachyon, and Arabidopsis had been furthermore investigated.

 

All these SRS proteins exhibited species-specific traits, together with monocot- and dicot-specific traits, as assessed by phylogenetic evaluation, which was additional validated by gene constructing and motif analyses. Genome comparisons revealed that segmental duplications may have carried out crucial roles contained in the recombination of the OsSRS gene household and their expression ranges. The household was primarily subjected to purifying selective stress.

 

Along with, the expression data demonstrated the distinct responses of OsSRS genes to varied abiotic stresses and hormonal cures, indicating their smart divergence. Our research gives an exquisite reference for elucidating the choices of SRS genes in rice.

 

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Ginsenoside Rg1 improves pathological damages by activating the p21‑p53‑STK pathway in ovary and Bax‑Bcl2 within the uterus in untimely ovarian insufficiency mouse fashions

The intention of the current research was to research the results of the ginsenoside Rg1 on D‑galactose (D‑gal)‑induced mouse fashions of untimely ovarian insufficiency (POI) and the associated mechanisms. C57BL/6 feminine mice had been randomly grouped into the next: i) D‑gal [subcutaneously (s.c.) 200 mg/kg/d D‑gal for 42 days]; ii) Rg1 [intraperitoneally (i.p.) 20 mg/kg/d Rg1 for 28 days]; iii) D‑gal + Rg1 (s.c. 200 mg/kg/d D‑gal for 42 days adopted by i.p. 20 mg/kg/d Rg1 for 28 days); and iv) saline teams (equal quantity of saline s.c. and that i.p.). Hematoxylin and eosin staining and electron microscopy had been used to research uterine and ovarian morphology.
Expression ranges of senescence components (p21, p53 and serine/threonine kinase), secretion of professional‑inflammatory cytokines [interleukin (IL)‑6, tumor necrosis factor (TNF)‑α and IL‑1β] and the actions of oxidation biomarkers [superoxide dismutase (T‑SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH‑px)] had been analyzed. The outcomes confirmed that mice within the Rg1 + D‑gal group had considerably larger uterine and ovarian weight in contrast with these within the D‑gal group.
Uterus morphology was additionally improved, primarily based on the comparability between the D‑gal group and the Rg1 + D‑gal group. As well as, the Rg1 remedy after D‑gal administration considerably decreased the expression of senescence‑related components, enhanced the actions of anti‑oxidant enzymes complete T‑SOD and GSH‑px along with decreasing TNF‑α, IL‑1β, MDA and IL‑6 (primarily based on the comparability between the D‑gal group and the Rg1 + D‑gal group).
In conclusion, the current research advised that the ginsenoside Rg1 improved pathological damages within the ovary and uterus by rising anti‑oxidant and anti‑inflammatory talents while decreasing the expression of senescence signaling pathways in POI mouse fashions.

 

 

PP1 (Src kinase inhibitor)

SIH-469-25MG 25 mg
EUR 619
  • PP1 is a potent and selective Src family protein tyrosine kinase inhibitor. This has a range of effects and implications. Structural studies have revealed that PP1 binds to the ATP-binding site in tyrosine kinases and Ser/Thr kinases. PP1 displays >
  • Show more
Description: The substance PP1 is a src kinase inhibitor. It is synthetically produced and has a purity of >98%. The pure substance is tan solid which is soluble in DMSO (25 mg/ml), slightly soluble in 100% ethanol.

PP1 (Src kinase inhibitor)

SIH-469-5MG 5 mg
EUR 205
  • PP1 is a potent and selective Src family protein tyrosine kinase inhibitor. This has a range of effects and implications. Structural studies have revealed that PP1 binds to the ATP-binding site in tyrosine kinases and Ser/Thr kinases. PP1 displays >
  • Show more
Description: The substance PP1 is a src kinase inhibitor. It is synthetically produced and has a purity of >98%. The pure substance is tan solid which is soluble in DMSO (25 mg/ml), slightly soluble in 100% ethanol.

PP2 (Src kinase inhibitor)

SIH-470-25MG 25 mg
EUR 737
  • PP2 is a selective inhibitor of Src-family tyrosine kinases. PP2 has been shown to inhibit Lck, FynT, and phosphorylation of focal adhesion kinase (FAK) and potently inhibits Hck, Lck (p56) and Fyn (p59). PP2 displays > 10000-fold selectivity over ZA
  • Show more
Description: The substance PP2 is a src kinase inhibitor. It is synthetically produced and has a purity of ?98%. The pure substance is off-white solid which is soluble in DMSO (25 mg/ml).

PP2 (Src kinase inhibitor)

SIH-470-5MG 5 mg
EUR 233
  • PP2 is a selective inhibitor of Src-family tyrosine kinases. PP2 has been shown to inhibit Lck, FynT, and phosphorylation of focal adhesion kinase (FAK) and potently inhibits Hck, Lck (p56) and Fyn (p59). PP2 displays > 10000-fold selectivity over ZA
  • Show more
Description: The substance PP2 is a src kinase inhibitor. It is synthetically produced and has a purity of ?98%. The pure substance is off-white solid which is soluble in DMSO (25 mg/ml).

SRC kinase signaling inhibitor 1 (SRCIN1) Antibody

abx331175-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.

SRC kinase signaling inhibitor 1 (SRCIN1) Antibody

20-abx327632
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

HPGDS inhibitor 1

B1046-1 1 mg
EUR 181
Description: HPGDS inhibitor 1 is an oral potent and selective inhibitor of hematopoietic prostaglandin D synthase (HPGDS) with IC50 value of 0.7nM [1].Prostaglandin D2 (PGD2) is a mediator of allergy and inflammation.

Anti-Src Rabbit Monoclonal Antibody

M00107-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Src Antibody. Validated in IF, WB and tested in Human, Rat.

Human SRC kinase signaling inhibitor 1, SRCIN1 ELISA KIT

ELI-13954h 96 Tests
EUR 824

Mouse SRC kinase signaling inhibitor 1, Srcin1 ELISA KIT

ELI-18126m 96 Tests
EUR 865

Rat SRC kinase signaling inhibitor 1 (SRCIN1) ELISA Kit

abx392007-96tests 96 tests
EUR 911
  • Shipped within 5-12 working days.

Human SRC kinase signaling inhibitor 1 (SRCIN1) ELISA Kit

abx385443-96tests 96 tests
EUR 911
  • Shipped within 5-12 working days.

Mouse SRC kinase signaling inhibitor 1 (SRCIN1) ELISA Kit

abx390644-96tests 96 tests
EUR 911
  • Shipped within 5-12 working days.

pp60 c-src (521-533) (phosphorylated)

B5235-1 1 mg
EUR 266

SRC 1 Antibody

abx238217-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

187-1, N-WASP inhibitor

B5251-1 1 mg
EUR 399

Srcin1 ELISA Kit| Mouse SRC kinase signaling inhibitor 1 ELISA

EF016287 96 Tests
EUR 689

YAP-TEAD Inhibitor 1 (Peptide 17)

A1149-1 1 mg
EUR 235

SRC - 1 (682–697)

5-01959 4 x 1mg Ask for price

SRC-1 (pT1179) Antibody

abx217070-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

anti- SRC 1 antibody

FNab08217 100µg
EUR 548.75
  • Recommended dilution: WB: 1:500-1:5000IHC: 1:20-1:200
  • Immunogen: nuclear receptor coactivator 1
  • Uniprot ID: Q15788
  • Gene ID: 8648
  • Research Area: Signal Transduction, Metabolism
Description: Antibody raised against SRC 1

T338C Src-IN-1

HY-16905 100mg
EUR 2943

Anti-SRC 1 antibody

PAab08217 100 ug
EUR 386

Srcin1 ELISA Kit| Rat SRC kinase signaling inhibitor 1 ELISA Ki

EF019367 96 Tests
EUR 689

Chk2 Inhibitor

1702-1
EUR 180

SCD1 Inhibitor

1716-1
EUR 218

ACC2 Inhibitor

1717-1
EUR 218

Syk Inhibitor

1983-1
EUR 126

Stat5 Inhibitor

9484-1
EUR 120

EGFR Inhibitor

C3327-1 1 mg
EUR 154
Description: EGFR inhibitor is a cell permeable, pyrimidine compound that selectively inhibits the EGFR kinase with IC50 value of 21 nM [1]. EGFR is a transmembrane protein, and is a receptor for members of epidermal growth factor family.

Lck Inhibitor

A3539-1 1 mg
EUR 154
Description: Lck Inhibitor is a small-molecule inhibitor of with IC50 value of 7 nM [1].The lymphocyte specific kinase which expressed in NK cells and T-cells is a member of the Src kinase family.

NFAT Inhibitor

A4539-1 1 mg
EUR 138
Description: Selective inhibitor of calcineurin-mediated dephosphorylation of nuclear factor of activated T cells (NFAT). Does not disrupt other calcineurin-dependent pathways. Inhibits NFAT activation and NFAT-dependent expression of endogenous cytokine genes in T cells.

c-SRC (c-SRC) Antibody

20-abx007517
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

c-SRC (c-SRC) Antibody

20-abx008688
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

Src/ Rat Src ELISA Kit

ELI-18125r 96 Tests
EUR 886

c-SRC (c-SRC) Antibody

abx232027-100ug 100 ug
EUR 551
  • Shipped within 5-12 working days.

c-SRC (c-SRC) Antibody

abx232028-100ug 100 ug
EUR 551
  • Shipped within 5-12 working days.

Phosphatase Inhibitor Cocktail 1 (100X in DMSO)

K1012-1 1 ml
EUR 102

NCOA1/SRC-1/KAT13A/ Rat NCOA1/ SRC- 1/ KAT13A ELISA Kit

ELA-E11292r 96 Tests
EUR 886

Human Plasminogen Activator Inhibitor-1 (PAI-1) AssayMax ELISA Kit

EP1100-1 96 Well Plate
EUR 417

SERPINE1 Plasminogen Activator Inhibitor-1 Human Recombinant Protein

PROTP05121-1 Regular: 25ug
EUR 317
Description: SERPINE1 Human Recombinant fused to N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 400 amino acids (24-402) and having a molecular mass of 45 kDa.;The SERPINE1 is purified by proprietary chromatographic techniques.

Bromodomain Inhibitor, (+)-JQ1

2070-1
EUR 164

Smad3 Inhibitor, SIS3

2227-1
EUR 164

CFTR Inhibitor-172

2487-1
EUR 120

EZSolution? SCD1 Inhibitor

2548-1
EUR 207

FAS Inhibitor, C75

1547-1
EUR 180

Batimastat (MMP Inhibitor)

1704-1
EUR 180

Cathepsin G Inhibitor

1982-1
EUR 240

USP7/USP47 Inhibitor

B2538-1
EUR 142

Cdk9 Inhibitor II

B2903-1
EUR 142

MLCK inhibitor peptide

B5236-1 1 mg
EUR 184

Rac1 Inhibitor W56

B5274-1 1 mg
EUR 399

Lyn peptide inhibitor

B5285-1 1 mg
EUR 601

Calpain Inhibitor XII

C5347-1 1 mg
EUR 145
Description: Ki: 19 nM for ?-calpain Calpain Inhibitor XII is a reversible and selective inhibitor of calpain I.Calpain is a protein belonging to the family of calcium-dependent, non-lysosomal cysteine proteases expressed ubiquitously in mammals and many other organisms.

MMP-13 Inhibitor

C4331-1 1 mg
EUR 187
Description: IC50: 8 nMMMP-13 Inhibitor is a MMP-13 inhibitor.Matrix metalloproteinases (MMPs), a family of zinc endopeptidases, can degrade proteins of the extracellular matrix, such as collagens, elastins, matrix glycoproteins, and proteoclycans.

EGFR/ErbB2 Inhibitor

C3285-1 1 mg
EUR 186
Description: IC50: 20 and 79 nM for EGFR and c-ErbB2, respectivelyEGFR/ErbB2 Inhibitor is an EGFR and c-ErbB2 inhibitor.EGFR and c-ErbB-2 are members of the epidermal growth factor receptor subfamily of protein tyrosine kinases.

MMP Inhibitor II

C4081-1 1 mg
EUR 119
Description: MMP Inhibitor II is a potent, reversible and broad-range inhibitor of matrix metalloproteinases (MMPs) with IC50 values of 24 nM, 18.4 nM, 30 nM and 2.7 nM for MMP-1, MMP-3, MMP-7 and MMP-9, respectively [1].

MEK Inhibitor Set

A9907-1 1 Set
EUR 224

Bromodomain Inhibitor, (+)-JQ1

A1910-1 1 mg
EUR 108
Description: Bromodomain Inhibitor, (+)-JQ1 is a potent and highly specific inhibitor for the BET (bromodomain and extra-terminal) family of bromodomains. (+)-JQ1 binds to BRD4 bromodomains 1 and 2 with Kd values of ~ 50 and 90 nM, respectively.

DiscoveryProbe? Inhibitor Library

L1048-.1 100 uL/well(10 mM solution)
EUR 14387

Plasminogen Activator Inhibitor 1 (PAI-1), hexapeptide, Human

SP-55196-1 0.5 mg
EUR 141

Src antibody

20R-2005 50 ug
EUR 281
Description: Rabbit polyclonal Src antibody

Src antibody

20R-2336 50 ug
EUR 281
Description: Rabbit polyclonal Src antibody

SRC antibody

70R-20514 50 ul
EUR 435
Description: Rabbit polyclonal SRC antibody

Src antibody

70R-13956 100 ug
EUR 322
Description: Affinity purified Rabbit polyclonal Src antibody

SRC Antibody

48186-100ul 100ul
EUR 333

SRC Antibody

48186-50ul 50ul
EUR 239

SRC Antibody

35933-100ul 100ul
EUR 252

SRC antibody

10R-2138 100 ul
EUR 457
Description: Mouse monoclonal SRC antibody

SRC antibody

10R-2154 100 ul
EUR 435
Description: Mouse monoclonal SRC antibody

Src antibody

10R-8485 100 ul
EUR 393
Description: Mouse monoclonal Src antibody

RR-SRC

5-01886 4 x 5mg Ask for price

Src Antibody

48902-100ul 100ul
EUR 333

Src Antibody

48902-50ul 50ul
EUR 239

Src Antibody

49317-100ul 100ul
EUR 333

Src Antibody

49317-50ul 50ul
EUR 239

SRC Antibody

1-CSB-PA001803
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/40000

SRC Antibody

1-CSB-PA001806
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

SRC Antibody

1-CSB-PA001807
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/10000

SRC Antibody

1-CSB-PA001809
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000

SRC Antibody

1-CSB-PA050134
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/5000

SRC Antibody

1-CSB-PA080378
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100

SRC Antibody

1-CSB-PA081645
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:15-1:50

src Antibody

1-CSB-PA24479A0Rb
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against src. Recognizes src from Zebrafish. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000

Src antibody

70R-37554 100 ug
EUR 273
Description: Rabbit Polyclonal Src antibody

Src antibody

70R-37603 100 ug
EUR 273
Description: Rabbit Polyclonal Src antibody

Src antibody

70R-32504 100 ug
EUR 327
Description: Rabbit polyclonal Src antibody

Src antibody

70R-32507 100 ug
EUR 327
Description: Rabbit polyclonal Src antibody

Src antibody

70R-32737 100 ug
EUR 327
Description: Rabbit polyclonal Src antibody

Src antibody

70R-34409 100 ug
EUR 327
Description: Rabbit polyclonal Src antibody
hicstatistics
Melting dsDNA Donor Molecules Vastly Improves Precision Genome Enhancing in Caenorhabditiselegans

CRISPR genome modifying has revolutionized genetics in quite a few organisms. Contained in the nematode Caenorhabditiselegans one injection into every of the 2 gonad arms of an grownup hermaphrodite exposes tons of of meiotic germ cells to modifying mixtures, allowing the restoration of a variety of indels or small precision edits from every successfully injected animal. Sadly, significantly for extended insertions, modifying efficiencies can differ broadly, necessitating a variety of injections, and customarily requiring co-selection methods.

 

Correct proper right here we present that melting double stranded DNA (dsDNA) donor molecules earlier to injection will enhance the frequency of tangible homology-directed restore (HDR) by a variety of fold for longer edits. We describe troubleshooting methods that let persistently excessive modifying efficiencies ensuing, as an illustration, in as rather a lot as 100 unbiased GFP knock-ins from a single injected animal. These efficiencies make C. elegans by far the perfect metazoan to genome edit, eradicating limitations to the use and adoption of this facile system as a mannequin for understanding animal biology.

 

Water-Pipe Smoking Publicity Deregulates a Set of Genes Related to Human Head and Neck Most cancers Improvement and Prognosis

  • Water-pipe smoking (WPS) is popping into in all probability probably the most well-liked kind of tobacco use among the many many many youth, notably contained in the Coronary heart East, altering cigarettes quickly and turning into a crucial danger of tobacco dependancy worldwide. Smoke from WPS accommodates comparable toxins as these current in cigarette smoke and is linked instantly with plenty of sorts of cancers together with lung and head and neck (HN) carcinomas.

 

  • Nonetheless, the underlying molecular pathways and/or goal genes accountable for the carcinogenic course of are nonetheless unknown. On this research, human widespread oral epithelial (HNOE) cells, NanoStringPanCancer Pathways panel of 770 gene transcripts and quantitative real-time polymerase chain response (qRT-PCR) evaluation had been utilized to hunt out differentially expressed genes (DEG) modulated by WPS. In silico evaluation was carried out to analysis the have an effect on of those genes in HN most cancers affected specific particular person’s biology and consequence. We discovered that WPS can induce the epithelial-mesenchymal transition (EMT: hallmark of most cancers development) of HNOE cells.

 

  • Additional considerably, our evaluation of NanoString revealed 23 genes deregulated beneath the have an effect on of WPS, accountable for the modulation of cell cycle, proliferation, migration/invasion, apoptosis, sign transduction, and inflammatory response. Further evaluation was carried out utilizing qRT-PCR of HNOE WPS-exposed and unexposed cells supported the reliability of our NanoString data.

 

  • Furthermore, we exhibit these DEG to be upregulated in most cancers in distinction with widespread tissue. Utilizing the Kaplan-Meier evaluation, we seen a major affiliation between WPS-deregulated genes and relapse-free survival/full survival in HN most cancers victims. Our findings level out that WPS can modulate EMT together with a set of genes which may very well be instantly concerned in human HN carcinogenesis, thereby affecting HN most cancers victims’ survival.
hicstatistics
hicstatistics

A pilot research on the genetic differ of Mycobacterium tuberculosis troublesome strains from tuberculosis victims contained in the Littoral area of Cameroon

Background: The re-emergence of tuberculosis (TB) worldwide, compounded by multi-drug resistance (MDR) of the causative brokers constitutes a major concern to the administration of the illness. Speedy analysis and correct stress identification are pivotal to the administration of the illness. This pilot research investigated the genetic differ of Mycobacterium tuberculosis troublesome (MTBC) strains from TB victims contained in the Littoral area of Cameroon together with their resistance to rifampicin (RIF).

 

Victims and strategies: This was a cross sectional hospital-based research carried out between January and December 2017 and together with 158 isolates from sputum smear constructive people [105 (66.5%) males and 53 (33.5%) females]. Sputum samples had been examined utilizing Xpert MTB/RIF, adopted by customized on Lowenstein-Jensen medium. Isolates had been additional subjected to molecular characterization utilizing IS6110 typing, deletion evaluation and spoligotyping.

 

Outcomes: 13 (8.8%) of the 147 isolates with susceptibility outcomes accessible had been proof in direction of RIF. Drug resistance occurred in 5/50 (10%) feminine in contrast with 8/97 (8.2%) male (OR, 0.81; 0.25-2.62; p = 0.764), and there was no essential distinction all by means of the age ranges (p = 0.448). Nonetheless, RIF resistance was related (OR, 0.18, 95%CI, 0.05-0.69; p = 0.023) with beforehand handled victims [(4/14 (28.6%)] in contrast with new ones [9/133 (6.8%)].

The 150 acknowledged lineages included amongst others 54 (36%) Cameroon, 18 (12%) UgandaI, 32 (21.3%) Haarlem, 17 (11.3%) Ghana, 9(6%) West African 1, 7(4.7%) Delhi/CAS, 4 (2.7%) LAM and three (2%) UgandaII. Of the 150 isolates, a really highly effective cluster was the Cameroon SIT 61, with 43(28.7%) isolates. Six (35.3%) of the 17 UgandaI sub-lineage had been RIF resistant (OR, 9.58; 95%CI, 2.74-33.55, p = 0.001).

 

Conclusion: The cosmopolitan Littoral area presents with a giant Mycobacterium tuberculosis (MTB) strains differ and the UgandaI sub-lineage attainable related to RIF resistance. Understanding the unfold of this clade by the use of surveillance will improve TB administration contained in the area.

 

Human Pim-1 Oncogene (PIM1) ELISA Kit
DLR-PIM1-Hu-96T 96T
EUR 673
  • Should the Human Pim-1 Oncogene (PIM1) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Pim-1 Oncogene (PIM1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Pim-1 Oncogene (PIM1) ELISA Kit
DLR-PIM1-Ra-48T 48T
EUR 549
  • Should the Rat Pim-1 Oncogene (PIM1) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Pim-1 Oncogene (PIM1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Pim-1 Oncogene (PIM1) ELISA Kit
DLR-PIM1-Ra-96T 96T
EUR 718
  • Should the Rat Pim-1 Oncogene (PIM1) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Pim-1 Oncogene (PIM1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Pim-1 Oncogene (PIM1) ELISA Kit
RDR-PIM1-Hu-48Tests 48 Tests
EUR 544
Human Pim-1 Oncogene (PIM1) ELISA Kit
RDR-PIM1-Hu-96Tests 96 Tests
EUR 756
Rat Pim-1 Oncogene (PIM1) ELISA Kit
RDR-PIM1-Ra-48Tests 48 Tests
EUR 583
Rat Pim-1 Oncogene (PIM1) ELISA Kit
RDR-PIM1-Ra-96Tests 96 Tests
EUR 811
Human Pim-1 Oncogene (PIM1) ELISA Kit
RD-PIM1-Hu-48Tests 48 Tests
EUR 521
Human Pim-1 Oncogene (PIM1) ELISA Kit
RD-PIM1-Hu-96Tests 96 Tests
EUR 723
Rat Pim-1 Oncogene (PIM1) ELISA Kit
RD-PIM1-Ra-48Tests 48 Tests
EUR 557
Rat Pim-1 Oncogene (PIM1) ELISA Kit
RD-PIM1-Ra-96Tests 96 Tests
EUR 775
Pim-1 Oncogene (PIM1) Antibody
20-abx101233
  • EUR 425.00
  • EUR 133.00
  • EUR 1205.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
Pim-1 Oncogene (PIM1) Antibody
20-abx101234
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
Pim-1 Oncogene (PIM1) Antibody
20-abx101235
  • EUR 453.00
  • EUR 133.00
  • EUR 1302.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.
Pim-1 Oncogene (PIM1) Antibody
abx146417-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.
Pim-1 Oncogene (PIM1) Antibody
20-abx174066
  • EUR 857.00
  • EUR 439.00
  • 1 mg
  • 200 ug
  • Please enquire.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse)
4-PAC578Mu01
  • EUR 251.00
  • EUR 2576.00
  • EUR 640.00
  • EUR 316.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1)
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat)
4-PAC578Ra01
  • EUR 259.00
  • EUR 2708.00
  • EUR 670.00
  • EUR 328.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr38~Leu177)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1)
Recombinant Pim-1 Oncogene (PIM1)
4-RPC578Hu01
  • EUR 467.36
  • EUR 228.00
  • EUR 1477.60
  • EUR 559.20
  • EUR 1018.40
  • EUR 376.00
  • EUR 3544.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P11309
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 47.8kDa
  • Isoelectric Point: 5.8
Description: Recombinant Human Pim-1 Oncogene expressed in: E.coli
Recombinant Pim-1 Oncogene (PIM1)
4-RPC578Mu01
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P06803
  • Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 17.1kDa
  • Isoelectric Point: 5.6
Description: Recombinant Mouse Pim-1 Oncogene expressed in: E.coli
Recombinant Pim-1 Oncogene (PIM1)
4-RPC578Ra01
  • EUR 467.36
  • EUR 228.00
  • EUR 1477.60
  • EUR 559.20
  • EUR 1018.40
  • EUR 376.00
  • EUR 3544.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P26794
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 17.4kDa
  • Isoelectric Point: Inquire
Description: Recombinant Rat Pim-1 Oncogene expressed in: E.coli
Pim-1 Oncogene (PIM1) Antibody (Biotin)
20-abx271779
  • EUR 453.00
  • EUR 244.00
  • EUR 1316.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.
Pim-1 Oncogene (PIM1) Antibody (Biotin)
20-abx272590
  • EUR 467.00
  • EUR 244.00
  • EUR 1344.00
  • EUR 634.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.
Pim-1 Oncogene (PIM1) Antibody (Biotin)
20-abx273089
  • EUR 481.00
  • EUR 244.00
  • EUR 1414.00
  • EUR 662.00
  • EUR 356.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), APC
4-PAC578Mu01-APC
  • EUR 351.00
  • EUR 3365.00
  • EUR 935.00
  • EUR 449.00
  • EUR 222.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with APC.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), Biotinylated
4-PAC578Mu01-Biotin
  • EUR 316.00
  • EUR 2526.00
  • EUR 744.00
  • EUR 387.00
  • EUR 221.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with Biotin.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), Cy3
4-PAC578Mu01-Cy3
  • EUR 427.00
  • EUR 4445.00
  • EUR 1205.00
  • EUR 557.00
  • EUR 254.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with Cy3.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), FITC
4-PAC578Mu01-FITC
  • EUR 301.00
  • EUR 2712.00
  • EUR 768.00
  • EUR 379.00
  • EUR 197.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with FITC.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), HRP
4-PAC578Mu01-HRP
  • EUR 321.00
  • EUR 2933.00
  • EUR 827.00
  • EUR 405.00
  • EUR 209.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with HRP.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), PE
4-PAC578Mu01-PE
  • EUR 301.00
  • EUR 2712.00
  • EUR 768.00
  • EUR 379.00
  • EUR 197.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with PE.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), APC
4-PAC578Ra01-APC
  • EUR 364.00
  • EUR 3545.00
  • EUR 980.00
  • EUR 467.00
  • EUR 227.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr38~Leu177)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with APC.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), Biotinylated
4-PAC578Ra01-Biotin
  • EUR 325.00
  • EUR 2658.00
  • EUR 777.00
  • EUR 400.00
  • EUR 225.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr38~Leu177)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Biotin.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), Cy3
4-PAC578Ra01-Cy3
  • EUR 444.00
  • EUR 4685.00
  • EUR 1265.00
  • EUR 581.00
  • EUR 261.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr38~Leu177)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Cy3.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), FITC
4-PAC578Ra01-FITC
  • EUR 311.00
  • EUR 2856.00
  • EUR 804.00
  • EUR 393.00
  • EUR 202.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr38~Leu177)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with FITC.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), HRP
4-PAC578Ra01-HRP
  • EUR 332.00
  • EUR 3089.00
  • EUR 866.00
  • EUR 421.00
  • EUR 213.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr38~Leu177)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with HRP.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), PE
4-PAC578Ra01-PE
  • EUR 311.00
  • EUR 2856.00
  • EUR 804.00
  • EUR 393.00
  • EUR 202.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr38~Leu177)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with PE.
Human Pim-1 Oncogene (PIM1) Protein
20-abx068546
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.
Rat Pim-1 Oncogene (PIM1) Protein
20-abx068547
  • EUR 648.00
  • EUR 272.00
  • EUR 1998.00
  • EUR 773.00
  • EUR 467.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.
Mouse Pim-1 Oncogene (PIM1) Protein
20-abx068548
  • EUR 662.00
  • EUR 272.00
  • EUR 2040.00
  • EUR 787.00
  • EUR 481.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat)
4-PAC578Hu01
  • EUR 247.00
  • EUR 2510.00
  • EUR 625.00
  • EUR 310.00
  • EUR 214.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr129~Leu268)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1)
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Mouse), APC-Cy7
4-PAC578Mu01-APC-Cy7
  • EUR 583.00
  • EUR 6610.00
  • EUR 1750.00
  • EUR 778.00
  • EUR 324.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr122~Leu261)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-1 Oncogene (PIM1). This antibody is labeled with APC-Cy7.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Rat), APC-Cy7
4-PAC578Ra01-APC-Cy7
  • EUR 608.00
  • EUR 6970.00
  • EUR 1840.00
  • EUR 814.00
  • EUR 335.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr38~Leu177)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-1 Oncogene (PIM1). This antibody is labeled with APC-Cy7.
Human Pim-1 Oncogene (PIM1)ELISA Kit
201-12-2345 96 tests
EUR 440
  • This Pim-1 Oncogene ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.
Human Pim-1 Oncogene (PIM1) CLIA Kit
20-abx190030
  • EUR 7911.00
  • EUR 4215.00
  • EUR 973.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.
Human Pim-1 Oncogene (PIM1) ELISA Kit
20-abx152744
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.
Human Pim-1 Oncogene (PIM1) ELISA Kit
abx573560-96tests 96 tests
EUR 668
  • Shipped within 5-12 working days.
Cow Pim-1 Oncogene (PIM1) ELISA Kit
abx519080-96tests 96 tests
EUR 911
  • Shipped within 5-12 working days.
Mouse Pim-1 Oncogene (PIM1) ELISA Kit
abx519082-96tests 96 tests
EUR 668
  • Shipped within 5-12 working days.
Rat Pim-1 Oncogene (PIM1) ELISA Kit
abx519083-96tests 96 tests
EUR 668
  • Shipped within 5-12 working days.
Human Pim-1 Oncogene(PIM1)ELISA Kit
QY-E04698 96T
EUR 361
Rat Pim-1 Oncogene(PIM1)ELISA Kit
QY-E10539 96T
EUR 400
Human Pim-1 Oncogene ELISA Kit (PIM1)
RK02083 96 Tests
EUR 521
Human Pim-1 Oncogene (PIM1)CLIA Kit
SCC578Hu-10x96wellstestplate 10x96-wells test plate
EUR 5647.8
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-1 Oncogene (PIM1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-1 Oncogene (PIM1) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Human Pim-1 Oncogene (PIM1)CLIA Kit
SCC578Hu-1x48wellstestplate 1x48-wells test plate
EUR 552.76
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-1 Oncogene (PIM1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-1 Oncogene (PIM1) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Human Pim-1 Oncogene (PIM1)CLIA Kit
SCC578Hu-1x96wellstestplate 1x96-wells test plate
EUR 746.8
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-1 Oncogene (PIM1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-1 Oncogene (PIM1) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Human Pim-1 Oncogene (PIM1)CLIA Kit
SCC578Hu-5x96wellstestplate 5x96-wells test plate
EUR 3060.6
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-1 Oncogene (PIM1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Pim-1 Oncogene (PIM1) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Human Pim-1 Oncogene (PIM1) CLIA Kit
4-SCC578Hu
  • EUR 5698.00
  • EUR 3061.00
  • EUR 747.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Pim-1 Oncogene Clia kit. Alternative names of the recognized antigen: Proto-Oncogene Serine/Threonine-Protein Kinase Pim-1
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Pim-1 Oncogene (PIM1)Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Human Pim-1 Oncogene (PIM1) ELISA Kit
SEC578Hu-10x96wellstestplate 10x96-wells test plate
EUR 4731.5
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-1 Oncogene (PIM1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-1 Oncogene (PIM1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Pim-1 Oncogene (PIM1) ELISA Kit
SEC578Hu-1x48wellstestplate 1x48-wells test plate
EUR 477.3
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-1 Oncogene (PIM1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-1 Oncogene (PIM1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Pim-1 Oncogene (PIM1) ELISA Kit
SEC578Hu-1x96wellstestplate 1x96-wells test plate
EUR 639
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-1 Oncogene (PIM1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-1 Oncogene (PIM1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Pim-1 Oncogene (PIM1) ELISA Kit
SEC578Hu-5x96wellstestplate 5x96-wells test plate
EUR 2575.5
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Pim-1 Oncogene (PIM1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Pim-1 Oncogene (PIM1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Pim-1 Oncogene (PIM1) ELISA Kit
4-SEC578Hu
  • EUR 4782.00
  • EUR 2526.00
  • EUR 640.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Pim-1 Oncogene elisa. Alternative names of the recognized antigen: Proto-Oncogene Serine/Threonine-Protein Kinase Pim-1
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Pim-1 Oncogene (PIM1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Mouse Pim-1 Oncogene(PIM1)ELISA Kit
QY-E21184 96T
EUR 361
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat), APC
4-PAC578Hu01-APC
  • EUR 345.00
  • EUR 3275.00
  • EUR 912.00
  • EUR 440.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr129~Leu268)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1). This antibody is labeled with APC.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat), Biotinylated
4-PAC578Hu01-Biotin
  • EUR 311.00
  • EUR 2460.00
  • EUR 727.00
  • EUR 381.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr129~Leu268)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Biotin.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat), Cy3
4-PAC578Hu01-Cy3
  • EUR 419.00
  • EUR 4325.00
  • EUR 1175.00
  • EUR 545.00
  • EUR 251.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr129~Leu268)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1). This antibody is labeled with Cy3.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat), FITC
4-PAC578Hu01-FITC
  • EUR 296.00
  • EUR 2640.00
  • EUR 750.00
  • EUR 372.00
  • EUR 195.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr129~Leu268)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1). This antibody is labeled with FITC.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat), HRP
4-PAC578Hu01-HRP
  • EUR 316.00
  • EUR 2855.00
  • EUR 807.00
  • EUR 398.00
  • EUR 206.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr129~Leu268)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1). This antibody is labeled with HRP.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat), PE
4-PAC578Hu01-PE
  • EUR 296.00
  • EUR 2640.00
  • EUR 750.00
  • EUR 372.00
  • EUR 195.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr129~Leu268)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1). This antibody is labeled with PE.
ELISA kit for Human PIM1 (Pim-1 Oncogene)
ELK3785 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pim-1 Oncogene (PIM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pim-1 Oncoge
  • Show more
Description: A sandwich ELISA kit for detection of Pim-1 Oncogene from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Pim-1 Oncogene (PIM1) Polyclonal Antibody (Human, Mouse, Rat), APC-Cy7
4-PAC578Hu01-APC-Cy7
  • EUR 571.00
  • EUR 6430.00
  • EUR 1705.00
  • EUR 760.00
  • EUR 319.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM1 (Tyr129~Leu268)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human, Mouse, Rat Pim-1 Oncogene (PIM1). This antibody is labeled with APC-Cy7.
Polyclonal PIM1 / Pim-1 Antibody (aa24-37)
APG01139G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human PIM1 / Pim-1 (aa24-37). This antibody is tested and proven to work in the following applications:
Rabbit Polyclonal antibody Anti-CRBN
Anti-CRBN 50 µg
EUR 349
Rabbit Pim 1 Oncogene ELISA kit
E04P0753-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Pim 1 Oncogene ELISA kit
E04P0753-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Pim 1 Oncogene ELISA kit
E04P0753-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Pim 1 Oncogene in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Proto-oncogene serine/threonine-protein kinase pim-1(PIM1) ELISA kit
CSB-E11825h-24T 1 plate of 24 wells
EUR 165
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Human Proto-oncogene serine/threonine-protein kinase pim-1 (PIM1) in samples from serum, plasma, tissue homogenates, cell culture supernates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Human Proto-oncogene serine/threonine-protein kinase pim-1(PIM1) ELISA kit
1-CSB-E11825h
  • EUR 804.00
  • EUR 5099.00
  • EUR 2704.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
  • Sample volume: 50-100ul
  • Detection wavelength: 450nm
  • Assay performance time: 1 to 4 hours.
Description: Quantitativesandwich ELISA kit for measuring Human Proto-oncogene serine/threonine-protein kinase pim-1(PIM1) in samples from serum, plasma, tissue homogenates, cell culture supernates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Serine/Threonine-Protein Kinase Pim-1 (PIM1) Antibody
20-abx214602
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.
Serine/Threonine-Protein Kinase Pim-1 (PIM1) Antibody
20-abx125380
  • EUR 495.00
  • EUR 704.00
  • EUR 356.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.
Serine/Threonine-Protein Kinase Pim-1 (PIM1) Antibody
abx033772-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.
Serine/Threonine-Protein Kinase Pim-1 (PIM1) Antibody
abx033772-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.
Serine/Threonine-Protein Kinase Pim-1 (PIM1) Antibody
20-abx241258
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.
Serine/Threonine-Protein Kinase Pim-1 (PIM1) Antibody
20-abx327418
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Serine/Threonine-Protein Kinase Pim-1 (PIM1) Antibody
20-abx302380
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.
Polyclonal Goat anti-GST α-form
GST-ANTI-1 50 uL
EUR 280
Pim-3 Oncogene (PIM3) Polyclonal Antibody (Human)
4-PAN637Hu01
  • EUR 262.00
  • EUR 2747.00
  • EUR 679.00
  • EUR 331.00
  • EUR 220.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM3 (Met1~Leu326)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Pim-3 Oncogene (PIM3)
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Human)
4-PAP797Hu01
  • EUR 262.00
  • EUR 2747.00
  • EUR 679.00
  • EUR 331.00
  • EUR 220.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM2 (Leu82~Glu291)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Pim-2 Oncogene (PIM2)
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Mouse)
4-PAP797Mu01
  • EUR 266.00
  • EUR 2813.00
  • EUR 694.00
  • EUR 337.00
  • EUR 222.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM2 (Gly98~Cys319)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Mouse Pim-2 Oncogene (PIM2)
Pim-2 Oncogene (PIM2) Polyclonal Antibody (Rat)
4-PAP797Ra01
  • EUR 275.00
  • EUR 2958.00
  • EUR 727.00
  • EUR 350.00
  • EUR 226.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PIM2 (Leu34~Glu292)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Pim-2 Oncogene (PIM2)
hicstatistics
Utilizing genome-scale fashions to optimize nutrient present for sustained algal progress and lipid productiveness
  • Nutrient availability is crucial for progress of algae and totally different microbes used for producing worthwhile biochemical merchandise. Determining the optimum ranges of nutrient offers to cultures can eliminate feeding of additional nutritional vitamins, decreasing manufacturing costs and reducing nutrient air air pollution into the environment.

 

  • With the looks of omics and bioinformatics methods, it is now attainable to assemble genome-scale fashions that exactly describe the metabolism of microorganisms. On this study, a genome-scale model of the inexperienced alga Chlorella vulgaris (iCZ946) was utilized to predict feeding of a lot of nutritional vitamins, along with nitrate and glucose, beneath every autotrophic and heterotrophic conditions.

 

  • The goal carry out was modified from optimizing progress to instead minimizing nitrate and glucose uptake prices, enabling predictions of feed prices for these nutritional vitamins. The metabolic model administration (MMC) algorithm was validated for autotrophic progress, saving 18% nitrate whereas sustaining algal progress.

 

  • Furthermore, we obtained comparable progress profiles by concurrently controlling glucose and nitrate offers beneath heterotrophic conditions for every extreme and low ranges of glucose and nitrate. Lastly, the nitrate present was managed in an effort to retain protein and chlorophyll synthesis, albeit at a lower charge, beneath nitrogen-limiting conditions.

 

  • This model-driven cultivation method doubled all the volumetric yield of biomass, elevated fatty acid methyl ester (FAME) yield by 61%, and enhanced lutein yield virtually Three fold compared with nitrogen starvation. This study introduces a administration methodology that integrates omics info and genome-scale fashions in an effort to optimize nutrient offers based mostly totally on the metabolic state of algal cells in a number of nutrient environments.

 

  • This technique might rework bioprocessing administration proper right into a strategies biology-based paradigm applicable for quite a lot of species in an effort to limit nutrient inputs, in the reduction of processing costs, and optimize biomanufacturing for the next expertise of fascinating biotechnology merchandise.

 

Melting dsDNA Donor Molecules Vastly Improves Precision Genome Enhancing in Caenorhabditiselegans

 

CRISPR genome modifying has revolutionized genetics in numerous organisms. Inside the nematode Caenorhabditiselegans one injection into each of the two gonad arms of an grownup hermaphrodite exposes tons of of meiotic germ cells to modifying mixtures, permitting the restoration of a lot of indels or small precision edits from each effectively injected animal. Sadly, considerably for prolonged insertions, modifying efficiencies can differ broadly, necessitating a lot of injections, and generally requiring co-selection strategies.

 

Proper right here we current that melting double stranded DNA (dsDNA) donor molecules earlier to injection will improve the frequency of tangible homology-directed restore (HDR) by a lot of fold for longer edits. We describe troubleshooting strategies that permit persistently extreme modifying efficiencies ensuing, as an illustration, in as a lot as 100 unbiased GFP knock-ins from a single injected animal. These efficiencies make C. elegans by far the best metazoan to genome edit, eradicating limitations to the use and adoption of this facile system as a model for understanding animal biology.

 

Longitudinal Assertion of Muscle Mass over 10 Years Consistent with Serum Calcium Ranges and Calcium Consumption amongst Korean Adults Aged 50 and Older: The Korean Genome and Epidemiology Analysis

 

The intention of this study was to analysis the longitudinal change in muscle mass over 10 years in line with serum calcium ranges and calcium consumption. An entire of 1497 males and 1845 women aged 50 years and older had been included. Very important muscle loss (SML) was outlined as a 5% or bigger loss from baseline, whereas time-dependent progress of SML was assessed in line with quartiles for corrected calcium stage and every day calcium consumption using Cox regression fashions.

 

The incidence of SML was 6.7 and 7.7 per 100-person-years amongst men and women, respectively. Groups with the underside corrected calcium ranges had additional excellent SML than these with elevated calcium ranges, irrespective of intercourse. The connection between SML and calcium consumption was very important solely amongst women. The hazard ratio for SML per 1 mmol/L improve in corrected calcium stage was 0.236 and 0.237 for men and women, respectively. In conclusion, low serum calcium ranges may predict SML amongst adults aged ≥ 50 years, whereas low calcium consumption is also a predictor for muscle loss amongst women. Subsequently, encouraging dietary calcium consumption amongst middle-aged and older adults for preservation of muscle mass must be thought-about.

hicstatistics
hicstatistics

Viral Bcl2s‘ transmembrane domain interact with host Bcl2 proteins to control cellular apoptosis

Viral control of programmed cell death relies in part on the expression of viral analogs of the B-cell lymphoma 2 (Bcl2) protein known as viral Bcl2s (vBcl2s). vBcl2s control apoptosis by interacting with host pro- and anti-apoptotic members of the Bcl2 family.
Here, we show that the carboxyl-terminal hydrophobic region of herpesviral and poxviral vBcl2s can operate as transmembrane domains (TMDs) and participate in their homo-oligomerization. Additionally, we show that the viral TMDs mediate interactions with cellular pro- and anti-apoptotic Bcl2 TMDs within the membrane.
Furthermore, these intra-membrane interactions among viral and cellular proteins are necessary to control cell death upon an apoptotic stimulus. Therefore, their inhibition represents a new potential therapy against viral infections, which are characterized by short- and long-term deregulation of programmed cell death.
miRNAs play an important role in the pathogenesis of intervertebral disc degeneration (IDD). The role and the underlying mechanism of miR-424-5p in human nucleus pulposus (NP) are still unknown. We aimed to explore the role of miR-424-5p in IDD. Real-time PCR was used to detect the expression of miR-424-5p and Bcl2 in IDD tissues and idiopathic scoliosis tissues.
Human NP cells were used in our study. MTT and Hoechst apoptosis assays were used to detect the proliferation and apoptosis of NP cells, respectively. Western blotting assays were used to detect the expression levels of Bcl-2, cleaved caspase-3, cleaved caspase-9, caspase-3 and caspase-9 in degenerative NP cells.
A luciferase reporter assay was applied to confirm the relationship between miR-424-5p and Bcl2. Our results showed that the expression of miR-424-5p was increased and Bcl2 was decreased in degenerative NP cells. miR-425-5p expression was negatively correlated with Bcl2 expression in IDD tissues.
Suppression of miR-424-5p using an inhibitor increased Bcl2 expression at both the mRNA and protein levels, and it promoted cell viability and inhibited apoptosis. Furthermore, the levels of cleaved caspase-3 and cleaved caspase-9 were downregulated in miR-424-5p-silenced NP cells. Interestingly, we found that silencing miR-424-5p increased p62 expression at both the mRNA and protein levels. Finally, a luciferase reporter assay verified the binding of the miR-424-5p and the 3’UTR of Bcl2. These results suggested that silencing miR-424-5p suppressed NP cell apoptosis by upregulating Bcl2. Therefore, miR-424-5p might be a novel target for IDD therapies.

 

Xpert Total Histone Extraction Kit
H7200-050 50 ext
EUR 361
Xpert Total Histone Extraction Kit
H7200-100 100 ext
EUR 509
EpiQuik Total Histone H3 Quantification Kit (Colorimetric)
P-3062 96 Assays
EUR 759.05
Description: Ask the seller for details
EpiQuik Total Histone H3 Quantification Kit (Fluorometric)
P-3063 96 Assays
EUR 788.05
Description: The best epigenetics products
EpiQuik Total Histone H4 Quantification Kit (Colorimetric)
P-3072 96 Assays
EUR 759.05
Description: reagents widely cited
EpiQuik Total Histone H4 Quantification Kit (Fluorometric)
P-3073 96 Assays
EUR 759.05
Description: Ask the seller for details
EpiQuik Nuclear Extraction Kit
OP-0002 100 Assays
EUR 292.15
Description: kits suitable for this type of research
EpiQuik Circulating Total Histone H3 Quantification Kit (Colorimetric)
P-3091 96 Assays
EUR 773.55
Description: kits suitable for this type of research
EpiQuik Whole Cell Extraction Kit
OP-0003 100 Extractions
EUR 270.4
Description: Ask the seller for details
EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric)
P-4030 96 Assays
EUR 773.55
Description: reagents widely cited
EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Fluorometric)
P-4031 96 Assays
EUR 788.05
Description: Ask the seller for details
EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Colorimetric)
P-4032 96 Assays
EUR 788.05
Description: The best epigenetics products
EpiQuik Total Histone H4 Acetylation Detection Fast Kit (Fluorometric)
P-4033 96 Assays
EUR 788.05
Description: kits suitable for this type of research
Total RNA Extraction Kit
K2014005 1 kit
EUR 344
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein Extraction Kit
K3011010 1 kit
EUR 370
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein Extraction Kit
BSP003 50Preps
EUR 109.16
  • Product category: Molecular Biology Kits/Protein - Extraction/Animal
Mammalian Total Protein Extraction Kit
20-abx098853
  • EUR 356.00
  • EUR 105.00
  • 100 ml
  • 2 ml
  • Shipped within 5-10 working days.
AnaPrep Total RNA Extraction Kit
Z1322015 1 kit (48 extractions) Including all required plastic disposables
EUR 308
Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications.
Human Total PSA (t-PSA) ELISA Kit
PRB-5049-TOTAL 96 assays
EUR 572
Total Protein Extraction Kit: TM Buffer
K3011010-1 13 ml
EUR 162
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Total Protein Extraction Kit: 50X PI
K3011010-2 260 ul
EUR 289
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.
Rapid Animal Total RNA Extraction Kit
AT4181 50Preps
EUR 88.06
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal
Rapid Animal Total RNA Extraction Kit
AT4182 250preps
EUR 202.25
  • Product category: Molecular Biology Kits/RNA - Extraction/Animal
EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free)
OP-0022 100 Extractions
EUR 292.15
Description: kits suitable for this type of research
AffiSelect Total Protein Extraction Solution
A0710-015 15X1ml
EUR 142
Bacterial Total Protein Extraction Reagent
abx090632-50100assays 50-100 assays
EUR 258
  • Shipped within 5-10 working days.
Plant Total Protein Extraction Reagent
abx090633-50100assays 50-100 assays
EUR 230
  • Shipped within 5-10 working days.
EpiQuik Histone H3 Citrullination ELISA Kit
P-3095 96 Assays
EUR 947.55
Description: reagents widely cited
EpiQuik Methyl-Histone H3K4 ChIP Kit
P-2007 48 Reactions
EUR 860.55
Description: fast delivery possible
EpiQuik Acetyl-Histone H3 ChIP Kit
P-2010 48 Reactions
EUR 875.05
Description: The best epigenetics products
EpiQuik Acetyl-Histone H4 ChIP Kit
P-2011 48 Reactions
EUR 875.05
Description: kits suitable for this type of research
EpiQuik Methyl-Histone H3K27 ChIP Kit
P-2015 48 Reactions
EUR 889.55
Description: The best epigenetics products
3-min Bacterial Total Protein Extraction Kit
P512L NULL
EUR 0
Human Total PSA (t-PSA) ELISA Kit
PRB-5049-TOTAL-5 5 x 96 assays
EUR 2283
RNA isolater Total RNA Extraction Reagent
R401-01 100 ml
EUR 133
EpiQuik Global Histone H3 Acetylation Assay Kit
P-4008 96 Assays
EUR 788.05
Description: kits suitable for this type of research
EpiQuik Global Histone H4 Acetylation Assay Kit
P-4009 96 Assays
EUR 860.55
Description: fast delivery possible
EpiQuik Tri-Methyl-Histone H3K9 ChIP Kit
P-2006 48 Reactions
EUR 875.05
Description: kits suitable for this type of research
EpiQuik Tissue Methyl-Histone H3K9 ChIP Kit
P-2008 48 Reactions
EUR 875.05
Description: reagents widely cited
EpiQuik Tissue Methyl-Histone H3K4 ChIP Kit
P-2009 48 Reactions
EUR 875.05
Description: Ask the seller for details
EpiQuik Tissue Acetyl-Histone H3 ChIP Kit
P-2012 48 Reactions
EUR 875.05
Description: fast delivery possible
EpiQuik Tissue Acetyl-Histone H4 ChIP Kit
P-2013 48 Reactions
EUR 875.05
Description: reagents widely cited
EpiQuik Tissue Methyl-Histone H3K27 ChIP Kit
P-2016 48 Reactions
EUR 889.55
Description: kits suitable for this type of research
EpiQuik Global Histone H3K4 Methylation Assay Kit
P-3017 96 Assays
EUR 889.55
Description: reagents widely cited
EpiQuik Global Histone H3K9 Methylation Assay Kit
P-3018 96 Assays
EUR 889.55
Description: Ask the seller for details
3-min Total Protein Extraction Kit (Animal cells)
P501 NULL
EUR 0
3-min Total Protein Extraction Kit (Animal cells)
P501L NULL
EUR 0
3-min Total Protein Extraction Kit (Animal cells)
P501S NULL
EUR 0
3-min Total Protein Extraction Kit (Animal Tissues)
P502 NULL
EUR 0
3-min Total Protein Extraction Kit (Animal Tissues)
P502L NULL
EUR 0
3-min Total Protein Extraction Kit (Animal Tissues)
P502S NULL
EUR 0
8-min Plant Tissue Total Protein Extraction Kit
P508L NULL
EUR 0
Total Protein Extraction Kit for Tendons (50 tests)
P5A04 NULL
EUR 0
Total Protein Extraction Kit for Muscles (50 tests)
P5A06 NULL
EUR 0
Total Protein Extraction Kit for Insects (50 tests)
P5A07 NULL
EUR 0
AnaPrep Total RNA Extraction Kit with DNase-Treatment
Z1322017 1 kit (48 extractions) Including all required plastic disposables
EUR 405
Description: This kit is developped for our fully automated magnetic bead-based nucleic acid extraction platform which uses preprogrammed protocols and can process up to 12 samples simultaneously. With the AnaPrep 12 Extractor you will have the option to choose and work with a wide range of sample and reagent volumes. This sturdy, realiable and user-friendly machine will save you both time and expenses while ensuring consistently high quality performance and nucleic acids for your downstream applications.
Extract-EZ E1, Total Subcellular Protein Extraction Kit
BSP073 50Preps
EUR 135.26
  • Product category: Molecular Biology Kits/Protein - Extraction/Animal
Histone/DNA Binding Protein Extraction Kit (cells)
P514-25 NULL
EUR 0
Histone/DNA Binding Protein Extraction Kit (cells)
P514-4 NULL
EUR 0
Histone/DNA Binding Protein Extraction Kit (cells)
P514-50 NULL
EUR 0
EpiQuik Circulating Histone H3 Citrullination ELISA Kit (Colorimetric)
P-3097 96 Assays
EUR 933.05
Description: The best epigenetics products
EpiQuik Circulating Monomethyl Histone H3K4 ELISA Kit (Colorimetric)
P-3108 96 Assays
EUR 773.55
Description: reagents widely cited
EpiQuik Circulating Dimethyl Histone H3K4 ELISA Kit (Colorimetric)
P-3110 96 Assays
EUR 773.55
Description: Ask the seller for details
EpiQuik Circulating Trimethyl Histone H3K4 ELISA Kit (Colorimetric)
P-3112 96 Assays
EUR 773.55
Description: The best epigenetics products
EpiQuik Circulating Monomethyl Histone H3K9 ELISA Kit (Colorimetric)
P-3114 96 Assays
EUR 773.55
Description: kits suitable for this type of research
EpiQuik Circulating Dimethyl Histone H3K9 ELISA Kit (Colorimetric)
P-3116 96 Assays
EUR 773.55
Description: fast delivery possible
EpiQuik Circulating Trimethyl Histone H3K9 ELISA Kit (Colorimetric)
P-3118 96 Assays
EUR 773.55
Description: reagents widely cited
EpiQuik Circulating Monomethyl Histone H3K27 ELISA Kit (Colorimetric)
P-3120 96 Assays
EUR 773.55
Description: Ask the seller for details
EpiQuik Circulating Dimethyl Histone H3K27 ELISA Kit (Colorimetric)
P-3122 96 Assays
EUR 773.55
Description: The best epigenetics products
EpiQuik Circulating Trimethyl Histone H3K27 ELISA Kit (Colorimetric)
P-3124 96 Assays
EUR 773.55
Description: kits suitable for this type of research
EpiQuik Circulating Monomethyl Histone H3K36 ELISA Kit (Colorimetric)
P-3126 96 Assays
EUR 773.55
Description: fast delivery possible
EpiQuik Circulating Dimethyl Histone H3K36 ELISA Kit (Colorimetric)
P-3128 96 Assays
EUR 773.55
Description: reagents widely cited
EpiQuik Circulating Trimethyl Histone H3K36 ELISA Kit (Colorimetric)
P-3130 96 Assays
EUR 773.55
Description: Ask the seller for details
EpiQuik Circulating Acetyl Histone H3K9 ELISA Kit (Colorimetric)
P-3132 96 Assays
EUR 773.55
Description: The best epigenetics products
EpiQuik Circulating Acetyl Histone H3K18 ELISA Kit (Colorimetric)
P-3134 96 Assays
EUR 773.55
Description: kits suitable for this type of research
EpiQuik Circulating Acetyl Histone H3K27 ELISA Kit (Colorimetric)
P-3136 96 Assays
EUR 773.55
Description: fast delivery possible
EpiQuik Circulating Acetyl Histone H3K56 ELISA Kit (Colorimetric)
P-3138 96 Assays
EUR 773.55
Description: reagents widely cited
EpiQuik Global Acetyl Histone H3K9 Quantification Kit (Colorimetric)
P-4010 96 Assays
EUR 788.05
Description: reagents widely cited
EpiQuik Global Acetyl Histone H3K9 Quantification Kit (Fluorometric)
P-4011 96 Assays
EUR 788.05
Description: Ask the seller for details
EpiQuik Global Acetyl Histone H3K14 Quantification Kit (Colorimetric)
P-4012 96 Assays
EUR 788.05
Description: The best epigenetics products
EpiQuik Global Acetyl Histone H3K14 Quantification Kit (Fluorometric)
P-4013 96 Assays
EUR 788.05
Description: kits suitable for this type of research
EpiQuik Global Acetyl Histone H3K18 Quantification Kit (Colorimetric)
P-4014 96 Assays
EUR 788.05
Description: fast delivery possible
EpiQuik Global Acetyl Histone H3K18 Quantification Kit (Fluorometric)
P-4015 96 Assays
EUR 788.05
Description: reagents widely cited
EpiQuik Global Acetyl Histone H3K23 Quantification Kit (Colorimetric)
P-4016 96 Assays
EUR 788.05
Description: Ask the seller for details
EpiQuik Global Acetyl Histone H3K23 Quantification Kit (Fluorometric)
P-4017 96 Assays
EUR 788.05
Description: The best epigenetics products
EpiQuik Global Acetyl Histone H3K36 Quantification Kit (Colorimetric)
P-4018 96 Assays
EUR 788.05
Description: kits suitable for this type of research
EpiQuik Global Acetyl Histone H3K36 Quantification Kit (Fluorometric)