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Enzymes in molecular biology Proteinase K, recombinant

Proteinase k, recombinant

Keep in mind

• The really useful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The preparation of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to Four M urea
• Ca2+ protects Proteinase Okay in route of autolysis, will enhance the thermal stability and has a regulatory perform for the substrate-binding website of Proteinase Okay
• Secure over an enormous pH differ 4. Zero to 12.5, optimum pH 7.5 to eight.0
• Optimum prepare at 50 to55°C
• Speedy denaturation of enzyme happens at temperatures above 65°C.

Proteinase kay – one in every of many sometimes used enzymes in molecular biology.

Superb totally different for protein digestion in assorted options.

  • BLIRT – expert in Proteinase Okay manufacturing for over 15 years.
  • Licensed manufacturing course of ISO 13485 ensuing all through the very best high-quality product with a really low batch-to-batch variability.
  • Big elements, the possibility of white labeling, and portioning service – liquid selection all through the quantity on the consumer’s request (as rather a lot as quite a lot of million samples month-to-month).

 

Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even through the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, fragrant, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Proteinase kay (Lyophilized)

For Basic Digestion of Protein in Pure Samples

  • Lively over a pH differ of 4.3–12.0, in 0.5% SDS or 1% Triton X-100
  • Purified to take away RNase and DNase actions

Secure at Room Temperature and Straightforward to Use

  • Lively over pH differ 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
  • Retains >80% prepare at temperatures as rather a lot as 60°C
  • No resuspension or thawing ahead of use
  • Cat.# MC5005, MC5008 provided at a highlight of 20mg/ml

 

Proteinase K, recombinant
Proteinase K, recombinant



Highlights

• Prepared-to-use reply
• Lively in a variety of response circumstances

Capabilities

• Isolation of genomic DNA from mouse tail
• Isolation of genomic DNA from cultured cells
• Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces
• Dedication of enzyme localization
• Enhancing cloning effectivity of PCR merchandise

Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It’s a broad-spectrum endopeptidase with a really excessive particular preparation.

Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in-depth purification to yield the best high-quality product.

Proteinase Okay Molecular Biology Grade (PCR Grade) is filled with life beneath a variety of response circumstances, together with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively utilizing for the digestion of proteins, together with DNases and RNases, all by nucleic acid preparations with out compromising the integrity of remoted DNA or RNA.

Decisions

  • Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
  • Excessive prepare and distinctive purity.
  • Lively at excessive temperatures (as rather a lot as 56 °C) and denaturing circumstances (e.g. all through the presence of urea and/or SDS), which makes it best for digesting proteins in quite a lot of options.
  • Secure over an enormous pH differ: 4.0–12.5 (optimum pH 7.5–8.0).
  • Decreased quantity of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
  • Accessible as powder, lyophilized “cake” or liquid.

Capabilities:

  • Extraction of DNA and RNA from fully completely totally different beginning offers.
  • Purification of goal provides from contaminating proteins.
  • Elimination of DNases and RNases all by nucleic acids isolation.

 

Proteinase K
GE0990-100MG 100 mg
EUR 94

Proteinase K
GE0990-1G 1 g
EUR 413

Proteinase K
GE0990-250MG 250 mg
EUR 154

Proteinase K
GE0990-25MG 25 mg
EUR 58

Proteinase K
GE0990-500MG 500 mg
EUR 249

Proteinase K
K1037-1 1 ml
EUR 67
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.

Proteinase K
K1037-5 5 ml
EUR 108
Description: Proteinase K is a broad-spectrum serine protease and our product is extracted from Pichia pastoris cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.

Proteinase K
P2180-010 100mg
EUR 182

Proteinase K
P2180-050 500mg
EUR 374

Proteinase K
P2180-100 1g
EUR 523

Proteinase K
Z5050002 100 mg
EUR 171
Description: No genetic downstream application can do without the good old Proteinase K.

Proteinase K protein
30R-2901 500 mg
EUR 425
Description: Purified recombinant Human Proteinase K protein

Proteinase K Antibody
6210-100
EUR 294

Proteinase K Antibody
6210-30T
EUR 146

PROTEINASE K SOLUTION
501-PK 5 ml
EUR 152
Description: Genomic PCR Grade Proteinase K Solution

Proteinase K Solution
BIO-37084 5ml @ 20mg/ml Ask for price

Proteinase K Solution
BIO-37085 5 x 5ml @ 20mg/ml Ask for price

Proteinase K solution
GZ5904-100ML 100 ml
EUR 907

Proteinase K solution
GZ5904-25ML 25 ml
EUR 349

10x Proteinase K
TG4039 0.5ml
EUR 134

Proteinase K - 1g
PB0451 1g
EUR 556.34

PROTEINASE K SOLUTION 10ML
502-PK 10 ml
EUR 239
Description: Genomic PCR Grade Proteinase K Solution

PROTEINASE K SOLUTION 25ML
503-PK 25 ml
EUR 502
Description: Genomic PCR Grade Proteinase K Solution

Proteinase K, 20mg/ml
P2170-002 2X1ml
EUR 151

Proteinase K, 20mg/ml
P2170-005 5X1ml
EUR 199

Proteinase K, Recombinant, Molecular Grade
9210-100
EUR 120

Proteinase K, Recombinant, Molecular Grade
9210-10G
EUR 2643

Proteinase K, Recombinant, Molecular Grade
9210-1G
EUR 338

Proteinase K, Recombinant, Molecular Grade
9210-500
EUR 262