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Quick willpower of retroviral titer

Whereas standard titration methods require as a lot as 10 days to complete, these titration kits require solely 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an infection, allowing you to hold out every on the equivalent day.

Virus Titration Kits

Our Titration Kits current a fast and straightforward approach for titrating your virus. The kits make use of each a quick RNA or DNA extraction step, and resolve viral RNA/DNA titer using qRT-PCR.

Advantages of abm’s titration kits:

  • No RNA purification — saves time and eliminates inaccuracy.
  • Ready-to-use reagent mix — reduces variability.
  • qRT-PCR in a single step — additional delicate and proper than completely different methods.
  • No NTC amplification — Distinctive primer design completely eliminates non-specific amplification in NTCs.

We moreover provide On the spot Lentivirus Titration Taking part in playing cards, which allow quick detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. Utilizing the lateral flow into immunoassay approach, viral titer yields are assessed instantly inside 10 minutes using our three step protocol.

he Retro-X qRT-PCR Titration Tools provides a fast and straightforward approach for measuring retrovirus titer. The kits use a quick RNA purification step sooner than quantifying the number of retroviral genome copies using qRT-PCR and TB Inexperienced utilized sciences.

 

Description

abm’s qPCR Retrovirus Titer Tools is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and might be completed beneath one hour. Designed to ship extreme sensitivity and specificity, the tools ensures minimal non-specific background and better complete effectivity as compared with comparable kits within the market. ROX reference dye is obtainable separate from the MasterMix, making this tools universally appropriate with most qPCR gadgets.

qPCR and One-Step RT MasterMixes
qPCR and One-Step RT MasterMixes

 

PRODUCT COMPONENT QUANTITY
BlasTaq 2X qPCR Titer MasterMix 1.25 ml
Primer Mix 100 rxn (200 µl)
Commonplace Administration DNA 50 µl
ROX Reference Dye 15 µl
Nuclease-Free H2O 2 x 1.zero ml

 

SKU G949
Unit quantity 100 rxn
Storage State of affairs Retailer at -20°C

 

Receive fixed retroviral transductions

A critical good thing about retroviral gene-delivery strategies is the facility to manage the extent of gene expression by adjusting the multiplicity of an an infection (MOI) to dictate the number of viral genomes that enter your objective cells. These titration kits allow you to make a quick correlation between the retroviral copy amount and retroviral infectivity in your cell type and in the long run allow you to administration the expression stage between experiments.

The best way it really works

The qRT-PCR course of entails subjecting serial dilutions of your sample and a administration RNA of acknowledged copy amount (provided) to one-step qRT-PCR. The retroviral copy amount contained in your sample can then be determined by evaluating its Ct value to the standard curve.

RNA purification and DNase I remedy steps are essential to the protocol so even viruses packaged using transient transfections, which always embrace residual plasmid DNA, might be exactly quantified using this tools.

 

Retrovirus Packaging Kit
P905 NULL
EUR 0
Retrovirus Mini Purification Kit
K1307-10
EUR 544
Retrovirus Mini Purification Kit
K1307-20
EUR 914
Retrovirus Maxi Purification Kit
K1308-10
EUR 1295
Retrovirus Maxi Purification Kit
K1308-2
EUR 533
Retrovirus Maxi Purification Kit
K1308-4
EUR 860
QuickTiter Retrovirus Quantitation Kit
VPK-120 20 assays
EUR 711
Description: Our QuickTiter Retrovirus Quantitation Kit provides a quick method for determining retroviral titer. This assay measures the viral nucleic acid content of your retrovirus, and may be performed either before or after purification of your virus.
M13 Phage Titration ELISA Kit
55R-PRPHAGE 96 tests
EUR 693
Description: Enzyme Immunoassay for the Quantitative Determination of purified M13 Bacteriophage Particles
Retrovirus Booster 50µl
ABP-RVC-RBST05 50µl Ask for price
    • Product line: Gryphon Retrovirus
    • Brand: Packaging System
Retrovirus Booster 200µl
ABP-RVC-RBST20 200µl Ask for price
    • Product line: Gryphon Retrovirus
    • Brand: Packaging System
Retrovirus Booster 400µl
ABP-RVC-RBST40 400µl Ask for price
    • Product line: Gryphon Retrovirus
    • Brand: Packaging System
Retrovirus Stabilizer 50µl
ABP-RVC-RSTB05 50µl Ask for price
    • Product line: Gryphon Retrovirus
    • Brand: Packaging System
Retrovirus Stabilizer 200µl
ABP-RVC-RSTB20 200µl Ask for price
    • Product line: Gryphon Retrovirus
    • Brand: Packaging System
Retrovirus Stabilizer 400µl
ABP-RVC-RSTB40 400µl Ask for price
    • Product line: Gryphon Retrovirus
    • Brand: Packaging System
Retro-SV40LT Retrovirus
RVP010 1.0 ml
EUR 247
Lentivirus and Retrovirus Purification Kit
K1458-100 100 Preps
EUR 1037
Lentivirus and Retrovirus Purification Kit
K1458-20 20 Preps
EUR 606
qPCR Control Template Kit
G929 100 rxns
EUR 148
Eva QPCR SuperMix Kit
K5052200 200 reactions
EUR 211
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Eva QPCR SuperMix Kit
K5052400 400 reactions
EUR 320
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Pro QPCR SuperMix Kit
K5053200 200 reactions
EUR 188
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
Pro QPCR SuperMix Kit
K5053400 400 reactions
EUR 299
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
QPCR Positive Control Kit
M1126-100
EUR 403
Lentivirus qPCR Quantification Kit
K1471-100 100 Rxns
EUR 298
Endogenous retrovirus (replication competent) PCR kit
PCR-V725-48D 50T
EUR 425.8
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)
Endogenous retrovirus (replication competent) PCR kit
PCR-V725-96D 100T
EUR 521.5
  • Contact us in order to know the reactivity of the kit.
Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent)
ViraDuctin Retrovirus Transduction Kit (40 Transductions)
RV-200 40 transductions
EUR 363
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.
ViraDuctin Retrovirus Transduction Kit (200 Transductions)
RV-201 200 transductions
EUR 780
Description: The efficiency of retrovirus infection of target cells tends to be lower than that of adenovirus. Additives such as Polybrene® can increase transduction efficiencies, but even then only a small fraction of retroviral vectors can trasduce many target cell lines. Our ViraDuctin Retrovirus Transduction Kit provides superior transduction efficiencies in a variety of cell lines, even when compared to transductions in the presence of Polybrene®. This system is ideal for many primary cells as well as immobilized cells.
Endogenous retrovirus (replication competent) PCR kit
PCR-H725-48D 48T
EUR 453