Search for:

RNase R shows low train on tRNA, rRNA and totally different extraordinarily structured RNAs, for which the three’ end is double stranded with a short 3’ overhang. These RNA species can stall the enzyme and finish in drastically lowered train. If inefficient degradation is seen, it is endorsed to each upscale the digestion, use additional RNase R, or take away rRNA from full RNA extracts earlier to digestion.

SAMPLE PROCESSING GUIDELINES AND TROUBLESHOOTING:

• For digestion of full RNA, longer incubations of 2-Three hours are generally required.
• If degradation is inefficient, use a barely elevated incubation temperature (40-45°C) and complement additional enzyme partway (e.g. 0.5 μl after 1 hour) by way of the method. The higher temperature is particularly useful for degrading extraordinarily structured linear RNAs, equal to rRNAs. Do not exceed 45°C or incubate over 3
hours, as this may end in non-enzymatic RNA degradation.

• Keep in mind that spherical RNAs symbolize a small proportion of full RNA (generally 0.1%-0.01%), because of this reality RNase R treatment will most actually finish in low ranges of RNA (picogram-range), presumably undetectable by most methods. For that purpose, a starting amount of not lower than 10 µg of full RNA is useful for a lot of downstream features.

• Whereas the enzyme could also be heat-inactivated the method should not be useful since extreme heat can lead to RNA hurt. Phenol-chloroform precipitation could be utilized instead. For NGS, robust half reversible immobilization (SPRI) bead cleanup is useful.

• Magnesium at concentrations of 0.1-1. Zero mM is required for optimum train. If ETA is
present, compensate by together with MgCl2 to 1. Zero Mm final focus.

Biovision rnase-r
Biovision rnase-r

DESCRIPTION:

RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species. This enzyme does not digest spherical, lariat, or double stranded RNA with temporary 3’ overhangs (decrease than sevennucleotides). As such, this enzyme is ideally suited to the study of lariat RNA produced by typical splicing, along with circRNAs which come up by way of back-splicing.

APPLICATIONS:
• Enriching circRNAs in natural samples
• Identification of intronic lariat sequences
• Identification of exonic circRNAs
• Studying varied splicing
• Manufacturing of artificial spherical RNAs

EZYME UNIT DEFINITION: One unit is printed as the amount of RNase R that converts 1
µg of poly(A) into acid soluble nucleotides in 10 minutes at 37°C. ENZYME STORAGE BUFFER: 50 mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol 10X RNase R REACTION BUFFER COMPONENTS: 200 mM Tris-HCl, 1 M KCl, 1 mM, MgCl2, pH 7.5.

STORAGE CONDITIONS: Retailer at -20°C.

Stay away from repeated freeze-thaw cycles of all components to retain most effectivity. All components
are regular for one 12 months from the date of transport when saved and handled appropriately.

RELATED PRODUCTS:

• RNase A (Cat# M1227-25)
• RNaseOFF ribonuclease Inhibitor (Cat# M1238-4000)
• DNase, E.coli DNA Ligase (Cat# M1217-100)
• Hyperlink-FAST 5 Minutes DNA Ligation Package deal (Cat# Okay902-50)
• New T4 DNA Ligase (Cat# M1247-200)
• T4 DNA Ligase (5 u/μl) (Cat# 9101-250)
• T4 RNA Ligase 1 (ssRNA Ligase) (Cat# M1218-100)
• T4 RNA Ligase 2 (dsRNA Ligase) (Cat# M1219-100)

Cat # +Dimension M1228-500
Dimension 500 U
Highlights RNase R is an E. coli exoribonuclease which shows 3’-to-5’ exonuclease train, successfully digesting virtually all linear RNA species.
Storage Conditions -20°C
Supply Conditions Gel Pack
USAGE For Evaluation Use Solely! Not For Use in Individuals.

 

RNase A

abx098139-1ml 1 ml
EUR 217
  • Shipped within 5-10 working days.

PI / RNAse

abx290029-200tests 200 tests
EUR 272
  • Shipped within 2-3 weeks.

RNase A

M1227-25
EUR 245

RNase A

RN103-001 300mg
EUR 2615

RNase A

RB0473 500mg
EUR 183.11
  • Product category: Proteins/Enzymes/RNase

RNAse H2A Antibody

24819-100ul 100ul
EUR 390

Rnase (90–105)

5-01885 4 x 1mg Ask for price

RNase A (MBG)

409-050 50 mg
EUR 73

RNase A (MBG)

409-250 250 mg
EUR 193

DNase (RNase-free)

abx098138-1500U 1500 U
EUR 453
  • Shipped within 5-10 working days.

RNase-Free water

abx098145-25ml 25 ml
EUR 175
  • Shipped within 5-10 working days.

RNase 1 Antibody

20-abx141499
  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.

RNase 13 Antibody

20-abx141582
  • EUR 370.00
  • EUR 606.00
  • EUR 300.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.

Rnase A, Pancreatic

abx082443-25mg 25 mg
EUR 189
  • Shipped within 5-10 working days.

RiboSafe RNase Inhibitor

BIO-65027 2500 Units Ask for price

RiboSafe RNase Inhibitor

BIO-65028 10,000 Units Ask for price

RNase T2 Antibody

abx237324-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.

RNase A Solution

G118 1.0 ml; 10 mg/ml
EUR 102

Thermostable RNAse H

M1224-100
EUR 321

RNase III, E.coli

M1226-100
EUR 224

Murine Rnase inhibitor

R301-01 2000 U
EUR 116

Murine Rnase inhibitor

R301-02 10000 U
EUR 166

Murine Rnase inhibitor

R301-03 20000 U
EUR 219

Rnase A, 1g

R9001-1g
EUR 822

Rnase A, 250mg

R9001-250mg
EUR 281

anti-RNase L

YF-PA24584 50 ul
EUR 334
Description: Mouse polyclonal to RNase L

anti-RNase H1

YF-PA26995 50 ul
EUR 334
Description: Mouse polyclonal to RNase H1

Ribunuclease Inhibitor / RNase Inhibitor

105-310 2000 u
EUR 76

Ribunuclease Inhibitor / RNase Inhibitor

105-350 10000 u
EUR 237

RNase III Drosha Antibody

45383-100ul 100ul
EUR 252

RNase III Drosha Antibody

45383-50ul 50ul
EUR 187

RNase III Drosha Antibody

DF8601 200ul
EUR 304
Description: RNase III Drosha Antibody detects endogenous levels of total RNase III Drosha.

RNase III Drosha Antibody

20-abx149918
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

PI / RNase Staining Buffer

20-abx090619
  • EUR 272.00
  • EUR 230.00
  • 100 tests
  • 50 tests
  • Shipped within 5-10 working days.

Human RNase ELISA Kit

EHR0364 96Tests
EUR 521

Bovine RNase ELISA Kit

EBR0364 96Tests
EUR 521

Anserini RNASE ELISA Kit

EAR0364 96Tests
EUR 521